Immunogenic response induced by wzm and wzt gene deletion mutants from Brucella abortus S19

文献类型: 外文期刊

第一作者: Wang, Xiu-Ran

作者: Wang, Xiu-Ran;Yan, Guang-Mou;Zhang, Rui;Wang, Xing-Long;Wang, Xiu-Ran;Zhang, Rui;Lang, Xu-Long;Li, Xiao-Yan;Chen, Si;Qian, Jing;Wang, Xing-Long;Wang, Xiu-Ran;Qian, Jing;Yang, Yan-Ling

作者机构:

关键词: ABC transporter;Brucellosis;Cytokine;ELISA;Rough mutant

期刊名称:MOLECULAR MEDICINE REPORTS ( 影响因子:2.952; 五年影响因子:2.754 )

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收录情况: SCI

摘要: Brucellosis is an infectious disease affecting humans and animals worldwide. Effective methods of control include inducing immunity in animals by vaccination and elimination. Brucella abortus S19 is one of the popular vaccines for control of cattle brucellosis, as it has low virulence. In this paper, allelic exchange plasmids of wzm and wzt genes were constructed and partially knocked out to evaluate the effects on the induction of immunity to Brucella abortus S19 mutants. Cytokine secretion in vitro, INF-γ induction in vivo and antibody dynamics were evaluated. These data suggested that the immunity-eliciting ability of the wzm and wzt gene deletion mutants was similar, although reduced compared with the S19 strain. The results demonstrated that the wzt gene may be more important in the regulation of the induction of immunity than the wzm gene.

分类号: R3

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