Positive inductive effect of swine interleukin-4 on immune responses elicited by modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine

文献类型： 外文期刊

第一作者： Peng, Jun

作者： Peng, Jun;Wang, Jinbao;Guo, Zhongkun;Wang, Jinbao;Wu, Jiaqiang;Du, Yijun;Li, Jun;Yu, Jiang;Xu, Shaojian;Zhang, Yuyu;Sun, Wenbo;Cong, Xiaoyan;Shi, Jianli;Peng, Jun;Wang, Jinbao;Wu, Jiaqiang;Du, Yijun;Li, Jun;Guo, Zhongkun;Yu, Jiang;Xu, Shaojian;Zhang, Yuyu;Sun, Wenbo;Cong, Xiaoyan;Shi, Jianli

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期刊名称：VIRAL IMMUNOLOGY （ 影响因子：2.257； 五年影响因子：1.882 ）

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收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome (PRRS) has become one of the most economically important diseases to the global pork industry. Currently, the efficacies of available commercial vaccines remain questionable: the modified live-PRRSV vaccines (MLVs) were generally effective but variable in sufficient protection, and the outcomes of inactivated-PRRSV vaccines (IVs) in the field were not very promising. In the present study, we investigated the effect of swine interleukin 4 (IL-4) on the development of virus-specific immune responses elicited by an MLV. The antibody titer against PRRSV membrane proteins in pigs elicited by MLV plus recombinant plasmid encoding IL-4 (group 3) was significantly higher than those elicited by MLV alone (group 1) and MLV plus empty plasmid (group 2) from 35 days post-inoculation (dpi). Similarly, the neutralizing efficacy of sera from group 3 was markedly enhanced compared with group 1 and group 2. In cellular immunity, the ratio of CD3+CD4 +/CD3+CD8+ T lymphocyte subpopulations from group 3 monitored by flow cytometry (FCM) was significantly higher than those from group1 and group 2 from 42 dpi to 21 days post-challenge (dpc). After viral challenge, pigs in group 3 showed significantly lower virus loads in peripheral blood measured by a real-time quantitative PCR (RT-qPCR), as compared with those in group 1 and group 2. Pigs in group 1 and group 2 had a low fever and displayed mild inappetence, lethargy, rough hair coats, and no lung lesions, while those in group 3 showed almost no clinical signs, no lung lesions. The scores of clinical signs of pigs in group 3 were significantly lower than those in both group 1 and group 2. Interestingly, the scores of lung lesions showed no significant differences among the three groups. Our results indicate that swine IL-4 markedly enhanced the protective immune response of pigs and improved the efficacy of the MLV in preventing PRRS disease.

分类号： R392

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