Construction and targeted retrieval of specific clone from a non-gridded soybean bacterial artificial chromosome library

文献类型： 外文期刊

第一作者： Xia, Zhengjun

作者： Xia, Zhengjun;Wu, Hongyan;Xia, Zhengjun;Watanabe, Satoshi;Harada, Kyuya

作者机构：

关键词： BAC;Pooling;Screening;Non-gridded library;Soybean

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

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收录情况： SCI

摘要： Although a post-genomic era is emerging for many plants, the bacterial artificial chromosome (BAC) library is still a valuable tool for genomic studies and preservation of precious genetic resources. Construction of non-gridded BAC libraries would dramatically reduce cost and save storage space. A non-gridded BAC library composed of approximately 96,000 insert-containing clones in 80 pools with an average insert size of 75 kb was constructed. This library represented 5.2 genome equivalents. We successfully developed a unique procedure to retrieve positive clones from the non-gridded pools. With this retrieving protocol, the non-gridded library system can be adapted to different species and to serve various research needs.

分类号： Q5`Q`O65

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