Cloning and characterization of a heme oxygenase-2 gene from rice (Oryza sativa L.), and its expression analysis in response to some abiotic stresses

文献类型： 外文期刊

第一作者： Wang, Lijuan

作者： Wang, Lijuan;Ma, Fei;Shen, Wenbiao;Xu, Sheng;Wang, Ren;Zheng, Tianqing;Chen, Huiping;Chen, Huiping

作者机构：

关键词： Abiotic stress responses;Gene expression;Heme oxygenase 2 (HO2);Prokaryotic expression;Rice (Oryza sativa L.);Subcellular localization

期刊名称：ACTA PHYSIOLOGIAE PLANTARUM （ 影响因子：2.354； 五年影响因子：2.711 ）

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年卷期：

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收录情况： SCI

摘要： Heme oxygenase (HO; EC 1.14.99.3) is an important enzyme that yields biliverdin IXα (BV), carbon monoxide and iron. At least two kinds of HO subfamilies exist in plants. Our previous report revealed that rice (Oryza sativa L.) HO1 (SE5, also named asOsHO1), a major subfamily of HO, is an oxidative stress–response protein, especially upon paraquat exposure. However, whether there exists rice HO2, another subfamily of HO, is still unknown. In the present study, a rice HO2 gene (named as OsHO2) was cloned and characterized. Its genomic sequence consists of four exons and three introns, and encodes a 36.5 kDa protein precursor with a 4.9 kDa N-terminal transit leader peptide. Further results showed that OsHO2 has a conserved HO signature sequence andshares a high amino acid sequence similarity with other identified plant HO2s. The recombinant mature OsHO2 (mOsHO2) protein expressed in Escherichia coli did not exhibit HO activity, which was in contrast with that of mOsHO1. The results of subcellularlocalization of OsHO2 demonstrated that it was most likely localized in the chloroplasts. Real-time RT-PCR experiment revealed that although OsHO2 mRNA is a much less abundant than that of OsHO1, both of them were expressed in all tested tissues. Importantly, OsHO2 transcripts could be differentially induced by hemin (a substrate of HO), paraquat (in particular), and NaCl treatments. Together, the results suggested that OsHO2 might act downstream in the signal transduction pathways following abiotic stresses in rice.

分类号： Q94

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