Rice black-streaked dwarf virus genome segment S5 is a bicistronic mRNA in infected plants

文献类型： 外文期刊

第一作者： Yang, Jian

作者： Yang, Jian;Liang-Ying, Dai;Yang, Jian;Zhang, Heng-Mu;Ying, Lu;Li, Jing;Lv, Ming-Fang;Xie, Li;Li, Pei-Pei;Liu, Xiao-Ya;Chen, Jian-Ping;Lv, Ming-Fang;Li, Pei-Pei;Liu, Xiao-Ya

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期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

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收录情况： SCI

摘要： Rice black-streaked dwarf virus (RBSDV) is a recognized member of the genus Fijivirus, family Reoviridae. Genome segment S5 has a putative second ORF partially overlapping the major ORF but in a different reading frame. This putative ORF is present in a published sequence and in two Chinese isolates now sequenced. Antibodies were raised against purified P5-1 and P5-2 fusion proteins expressed in a prokaryotic system. In western blots, these antibodies reacted with proteins of about 106 and 27 kDa, respectively, as predicted by sequence analysis. In immunoelectron microscopy, antibodies to P5-1 reacted with viroplasms, indicating that P5-1 is a component of viroplasms, but no labeling was observed with P5-2 antisera. Northern blot assays showed that the genome segment S5 was transcribed as a single mRNA with no subgenomic RNA. These results show that S5 is functionally bicistronic in infected plants. Possible translational mechanisms for P5-2 are discussed.

分类号： R37

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