Calcium-calmodulin dependent protein kinase I from Macrobrachium nipponense: CDNA cloning and involvement in molting
文献类型: 外文期刊
第一作者: Shen, Huaishun
作者: Shen, Huaishun;Hu, Yacheng;Zhang, Yuanqin;Zhou, Xin;Xu, Zenghong;Shen, Huaishun;Hu, Yacheng;Zhang, Yuanqin
作者机构:
关键词: Calcium-calmodulin-dependent protein kinase I;DsRNA-mediated RNA interference;Eyestalk ablation;Macrobrachium nipponense;Molting
期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Calcium-calmodulin dependent protein kinase I is a component of a calmodulin-dependent protein kinase cascade and involved in many physiological processes. The full-length cDNA of calcium-calmodulin dependent protein kinase I (MnCaMKI) was cloned from the freshwater prawn Macrobrachium nipponense and its expression pattern during the molt cycle and after eyestalk ablation is described. The full-length cDNA of MnCaMKI is 3262. bp in length and has an open reading frame (ORF) of 1038. bp, encoding a 345 amino acid protein. The expression of MnCaMKI in three examined tissues was upregulated in the premolt stage of the molt cycle. Its expression was induced after eyestalk ablation (ESA): the highest expression level was reached 1. day after ESA in hepatopancreas, and 3. days after ESA in muscle. By dsRNA-mediated RNA interference assay, expression of MnCaMKI and ecydone receptor gene (MnEcR) was significantly decreased in prawns treated by injection of dsMnCaMKI, while expression of these two genes was also significantly decreased in prawns treated by injection of dsMnEcR, demonstrating a close correlation between the expression of these two genes. These results suggest that CaMKI in M. nipponense is involved in molting.
分类号: R394
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