Molecular characterization, transcriptional regulation and association analysis with carcass traits of porcine TCAP gene
文献类型: 外文期刊
第一作者: Qiao, Mu
作者: Qiao, Mu;Wu, Huayu;Wu, Junjing;Peng, Xianwen;Mei, Shuqi;Huang, Jingshu
作者机构:
关键词: Pig;Promoter;SNP;TCAP;Transcriptional analysis
期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )
ISSN:
年卷期:
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收录情况: SCI
摘要: TCAP (also known as titin-cap or telethonin) is one of the titin interacting Z-disk proteins involved in the regulation and development of normal sarcomeric structure. In this study, we cloned the cDNA and promoter sequences of porcine TCAP gene, which contained a 504. bp full-length coding region. Quantitative real-time PCR (qRT-PCR) analyses showed that porcine TCAP was highly expressed in the skeletal muscle, heart, and kidney. During postnatal muscle development, TCAP expression was down-regulated from 30. days to 120. days in Large White and Meishan pigs. One single nucleotide polymorphism c.334G>A in exon 2 of the TCAP gene was identified and detected by allele-specific primer-polymerase chain reaction (ASP-PCR). Association analysis revealed that the polymorphism had significant associations (P<. 0.05 and P<. 0.01) with some carcass traits. Analysis of the porcine TCAP promoter in different cell lines demonstrated that it is a muscle-specific promoter. In addition, we found that the porcine TCAP promoter can be activated by MyoD, MyoG and MEF2 in myotubes, which indicated that TCAP may play a role in the regulation of porcine skeletal muscle development. These findings provide useful information for the further investigation of the function of TCAP in porcine skeletal muscle.
分类号: R394
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