Molecular characterization and expression patterns of myogenin in compensatory growth of Megalobrama amblycephala

文献类型: 外文期刊

第一作者: Zhu, Kecheng

作者: Zhu, Kecheng;Chen, Liping;Zhao, Jinkun;Wang, Huijuan;Wang, Weimin;Wang, Huanling;Zhu, Kecheng;Zhao, Jinkun;Wang, Huijuan;Wang, Weimin;Wang, Huanling;Li, Zhong

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关键词: Megalobrama amblycephala;Myogenin;Compensatory growth;Starvation/refeeding

期刊名称:COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY ( 影响因子:2.231; 五年影响因子:2.215 )

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收录情况: SCI

摘要: Myogenin (myog) is a muscle-specific basic helix–loop–helix (bHLH) transcription factor that plays an essential role in regulating skeletal muscle development and growth. To investigate molecular characterization of myog and the effect of starvation/refeeding on the gene expression,we isolated the myog cDNA sequence and analyzed the expression patterns using quantitative real-time polymerase chain reaction in Megalobrama amblycephala. Sequence analysis indicated thatM. amblycephalamyog shared an analogous structurewith the highly conserved His/Cys-rich, bHLHand C-terminal helix III domainswith other vertebrates. Sequence alignment and phylogenetic tree showed that M. amblycephala myog had the highest identity with the homologues of Ctenopharyngodon idella and Cyprinus carpio. Spatio-temporal expression patterns revealed that myog mRNA levels at the segmentation period and 12 h post-hatching (hph) were significantly higher than at other development stages (P < 0.05). Furthermore, the highest myog expression level was predominantly observed in white muscle compared with the other types of muscle. Fish body weight continuously decreased during 21-day starvation and then significantly increased after 7 days of refeeding and reached the similar level to the control at 21 days of refeeding, indicating that the pattern of complete compensatory growth possibly occurred in M. amblycephala; meanwhile, the relative somatic growth rate after refeeding was also dramatically higher than the control group. In addition, the myog expression decreased during 21 days of starvation and then exhibited a strong rebound effect after 7 days of refeeding and subsequently declined gradually to the control level by 21 days of refeeding.

分类号: Q5`Q6

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