Changes in biosynthesis and metabolism of glutathione upon ochratoxin A stress in Arabidopsis thaliana

文献类型： 外文期刊

第一作者： Wang, Yan

作者： Wang, Yan;Zhao, Weiwei;Hao, Junran;Xu, Wentao;Luo, YunBo;Wu, Weihong;Huang, Kunlun;Xu, Wentao;Luo, YunBo;Yang, Zhuojun;Liang, Zhihong;Huang, Kunlun;Wang, Yan

作者机构：

关键词： Ochratoxin A;Arabidopsis thaliana;Glutathione;Glutamateecysteine ligase;Glutathione-S-transferase;Glutathione peroxidase;Glutathione reductase

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Ochratoxin A (OTA) is one of the most toxic mycotoxins, which is toxic to plants and simulates oxidative stress. Glutathione is an important antioxidant in plants and is closely associated with detoxification in cells. We have previously shown that OTA exposure induces obvious expression differences in genes associated with glutathione metabolism. To characterize glutathione metabolism and understand its role in OTA phytotoxicity, we observed the accumulation of GSH in the detached leaves of Arabidopsis thaliana under OTA treatment. OTA stimulated a defense response through enhancing glutathione-S-transferase, glutathione peroxidase, glutathione reductase activities, and the transcript levels of these enzymes were increased to maintain the total glutathione content. Moreover, the level of oxidized glutathione (GSSG) was increased and the ascorbateeglutathione cycle fluctuated in response to OTA. The depletion of glutathione using buthionine sulfoximine (BSO, inhibitor of glutamateecysteine ligase) had no profound effect on OTA toxicity, as glutathione was regenerated through the ascorbateeglutathione cycle to maintain the total glutathione content. The ROS, MDA and GSH accumulation was significantly affected in the mutant gsh1, gr1 and gpx2 after treatment with OTA, which indicated that glutathione metabolism is directly involved in the oxidative stress response of Arabidopsis thaliana subjected to OTA. In conclusion, date demonstrate that glutathione-associated metabolism is closely related with OTA stress and glutathione play a role in resistance of Arabidopsis subjected to OTA.

分类号： Q945`Q946

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