The effect of myostatin silencing by lentiviral-mediated RNA interference on goat fetal fibroblasts

文献类型： 外文期刊

第一作者： Lu, Jian

作者： Lu, Jian;Wei, Caihong;Zhang, Xiaoning;Xu, Lingyang;Zhang, Shifang;Liu, Jiasen;Cao, Jiaxue;Zhao, Fuping;Zhang, Li;Du, Lixin;Lu, Jian;Lu, Jian;Li, Bichun;Liu, Jiasen;Cao, Jiaxue

作者机构：

关键词： Myostatin;Lentiviral;RNA interference;Fibroblasts;Myogenesis;Adipogenesis

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Myostatin is a transforming growth factor-beta family member that acts as a negative regulator of skeletal muscle mass. To identify possible myostatin inhibitors that may promote muscle growth, we used RNA interference mediated by a lentiviral vector to knockdown myostatin in goat fetal fibroblast cells. We also investigated the expression changes in relevant myogenic regulatory factors (MRFs) and adipogenic regulatory factors in the absence of myostatin in goat fetal fibrolasts. Quantitative RT-PCR revealed that myostatin transcripts were significantly reduced by 75 % (P < 0.01). Western blot showed that myostatin protein expression was reduced by 95 % (P < 0.01). We also found that the mRNA expression of activin receptor IIB (ACVR2B) significantly increased by 350 % (P < 0.01), and p21 increased 172 % (P < 0.01). Furthermore, myostatin inhibition decreased Myf5 and increased MEF2C mRNA expression in goat fetal fibroblasts, suggesting that myostatin regulates MRFs differently in fibroblasts compared to muscle. In addition, the expression of adipocyte marker genes peroxisome proliferator-activated receptor (PPAR) gamma and leptin, but not CCAAT/enhance-binding protein (C/EBP) alpha and C/EBP beta, were upregulated at the transcript level after myostatin silencing. These results suggest that we have generated a novel way to block myostatin in vitro, which could be used to improve livestock meat production and gene therapy of musculoskeletal diseases. This also suggests that myostatin plays a negative role in regulating the expression of adipogenesis related genes in goat fetal fibroblasts.

分类号： Q7

  • 相关文献

[1]Transcript characteristic of myostatin in sheep fibroblasts. Lu, Jian,Ding, Jiatong,Li, Bichun,Lu, Jian,Zhang, Xiaoning,Zhao, Fuping,Zhou, Xinlei,Zhang, Li,Wei, Caihong,Du, Lixin,Ren, Hangxing,Sheng, Xihui,Li, Shangang.

[2]Selection of an effective small interference RNA to silence myostatin gene expression in sheep fibroblast cells. Lu, Jian,Ding, Jiatong,Li, Bichun,Lu, Jian,Xu, Lingyang,Lu, Guobin,Zhao, Fuping,Wei, Caihong,Zhang, Li,Du, Lixin,Sun, Dan.

[3]Identification of Histone Deacetylase 2 as a Functional Gene for Skeletal Muscle Development in Chickens. Shahjahan, Md.,Wang, Fangjie,Liu, Ranran,Zhao, Guiping,Zheng, Maiqing,Zhang, Jingjing,Song, Jiao,Wen, Jie.

[4]Molecular cloning and characterization of the Myf5 gene in sea perch (Lateolabrax japonicus). Ye, Han-Qing,Chen, Song-Lin,Xu, Jian-Yong.

[5]Lead induces Siberian tiger fibroblast apoptosis by interfering with intracellular homeostasis. Liu, Zheng,Wang, Hui,Guo, Xuesong,Zhang, Wenxiu,Yuan, Ziao,Yuan, Hongyi,Liu, Xueting,Guan, Weijun,Zhang, Minghai. 2018

[6]Reversine Increases the Plasticity of Long-Term Cryopreserved Fibroblasts to Multipotent Progenitor Cells through Activation of Oct4. Li, Xiangchen,Guo, Yu,Yao, Yaxin,Ma, Yuehui,Liu, Changqing,Guan, Weijun,Guo, Yu,Liu, Changqing,Hua, Jinlian. 2016

[7]Establishment and biological characteristics of a jingning chicken embryonic fibroblast bank. Bai, C.,Jin, D.,Li, C.,Guan, W.,Ma, Y.,Wang, D.,Li, C..

[8]Establishment and Characterization of a Fibroblast Line from Duroc. Xiong, Hui,He, Xiaohong,Zhang, Wenxiu,Li, Changli,Li, Mei,Guan, Weijun,Ma, Yuehui,Xiong, Hui. 2014

[9]Overexpression of SIRT6 in Porcine Fetal Fibroblasts Attenuates Cytotoxicity and Premature Senescence Caused by D-Galactose and Tert-Butylhydroperoxide. Xie, Xiaoxian,Zhang, Hao,Wang, Liangliang,Zhang, Ailing,Li, Jiaqi,Xie, Xiaoxian,Zhang, Hao,Wang, Liangliang,Zhang, Ailing,Li, Jiaqi,Gao, Ping,Xie, Shuihua.

[10]Optimization of the Transfection Efficiency of Buffalo Fetal Fibroblasts Cells Using Liposome. Deng, Tingxian,Pang, Chunying,Zhang, Yingyin,Yang, Bingzhuang,Liang, Xianwei. 2013

[11]Generation of porcine fetal fibroblasts expressing the tetracycline-inducible Cas9 gene by somatic cell nuclear transfer. Liu, Guoqian,Liu, Kai,Wei, Hengxi,Li, Li,Zhang, Shouquan,Liu, Guoqian,Liu, Kai,Wei, Hengxi,Li, Li,Zhang, Shouquan.

[12]Apoptosis Induced by Duck Reovirus p10.8 Protein in Primary Duck Embryonated Fibroblast and Vero E6 Cells. Geng, Hongwei,Zhang, Yun,Guo, Dongchun,Wang, Yu,Liu, Ming,Zhang, Yun,Liu-Partanen, Yin,Tong, Guangzhi.

[13]Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig. Hua, Z.,Liu, X.,Bi, Y.,Xiao, H.,Hua, W.,Li, L.,Zhang, L.,Ren, H.,Zheng, X.,Xu, G..

[14]Suppressive Effects of Quercetin-3-O-(6?-Feruloyl)-ss-D-Galactopyranoside on Adipogenesis in 3T3-L1 Preadipocytes Through Down-regulation of PPAR? and C/EBPa Expression. Yang, Lei,Zhang, Ya-Ou,Cai, Guo-Ping,Yang, Lei,Zhang, Ya-Ou,Cai, Guo-Ping,Li, Xiao-Fan,Gao, Lei. 2012

[15]Oleate promotes differentiation of chicken primary preadipocytes in vitro. Shang, Zhouchun,Guo, Lin,Wang, Ning,Shi, Hui,Wang, Yuxiang,Li, Hui,Shang, Zhouchun,Guo, Lin,Wang, Ning,Shi, Hui,Wang, Yuxiang,Li, Hui,Shang, Zhouchun,Guo, Lin,Wang, Ning,Shi, Hui,Wang, Yuxiang,Li, Hui. 2014

[16]Silymarin inhibits adipogenesis in the adipocytes in grass carp Ctenopharyngodon idellus in vitro and in vivo. Xiao, Peizhen,Yang, Zhou,Sun, Jian,Tian, Jingjing,Chang, Zhiguang,Li, Xuexian,Ji, Hong,Zhang, Baotong,Ye, Yuantu,Yu, Ermeng,Xie, Jun.

[17]Anti-adipogenic Effects of Polyphenol Extracts of Areca Flower Tea on 3T3-L1 Preadipocytes. Song, Fei,Tang, MinMin,Shen, XiaoJun,Wang, Hui,Chen, Hua,Zhao, SongLin,Wu, QiuSheng.

[18]Expression of adenosine monophosphate-activated protein kinase subunit related to the rate of intramuscular lipogenesis in fast and slow-growing chicken strains. Yang, Ye,Song, Jiao,Fu, Rui-Qi,Sun, Yan-Fa,Wen, Jie.

[19]Cloning, Expression and Function Analysis of Chicken FBXO38t1. Zhang Zhi-Wei,Sun Ying-Ning,Rong En-Guang,Li Hui,Wang Ning,Zhang Zhi-Wei,Sun Ying-Ning,Rong En-Guang,Li Hui,Wang Ning,Zhang Zhi-Wei,Sun Ying-Ning,Rong En-Guang,Li Hui,Wang Ning,Sun Ying-Ning. 2013

[20]Epigenetic regulation of adipocyte differentiation and adipogenesis. Li, Hong-xing,Xiao, Lei,Gao, Jia-li,Zhai, Yong-gong,Xiao, Lei,Gao, Jia-li,Zhai, Yong-gong,Xiao, Lei,Gao, Jia-li,Zhai, Yong-gong,Xiao, Lei,Gao, Jia-li,Zhai, Yong-gong,Wang, Cheng. 2010

作者其他论文 更多>>

微信小程序