The association of SNPs in Hsp90 beta gene 5 ' flanking region with thermo tolerance traits and tissue mRNA expression in two chicken breeds

文献类型： 外文期刊

第一作者： Chen, Zhuo-Yu

作者： Chen, Zhuo-Yu;Gan, Jian-Kang;Xiao, Xiong;Jiang, Li-Yan;Zhang, Xi-Quan;Luo, Qing-Bin;Chen, Zhuo-Yu;Gan, Jian-Kang;Xiao, Xiong;Jiang, Li-Yan;Zhang, Xi-Quan;Luo, Qing-Bin

作者机构：

关键词： Hsp90β gene;Polymorphism;Heat stress;Quantitative real-time PCR;Chicken

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： Thermo stress induces heat shock proteins (HSPs) expression and HSP90 family is one of them that has been reported to involve in cellular protection against heat stress. But whether there is any association of genetic variation in the Hsp90 beta gene in chicken with thermo tolerance is still unknown. Direct sequencing was used to detect possible SNPs in Hsp90 beta gene 5' flanking region in 3 chicken breeds (n = 663). Six mutations, among which 2 SNPs were chosen and genotypes were analyzed with PCR-RFLP method, were found in Hsp90 beta gene in these 3 chicken breeds. Association analysis indicated that SNP of C.-141G > A in the 5' flanking region of the Hsp90 beta gene in chicken had some effect on thermo tolerance traits, which may be a potential molecular marker of thermo tolerance, and the genotype GG was the thermo tolerance genotype. Hsp90 beta gene mRNA expression in different tissues detected by quantitative real-time PCR assay were demonstrated to be tissue dependent, implying that different tissues have distinct sensibilities to thermo stress. Besides, it was shown time specific and varieties differences. The expression of Hsp90 beta mRNA in Lingshan chickens in some tissues including heart, liver, brain and spleen were significantly higher or lower than that of White Recessive Rock (WRR). In this study, we presume that these mutations could be used in marker assisted selection for anti-heat stress chickens in our breeding program, and WRR were vulnerable to tropical thermo stress whereas Lingshan chickens were well adapted.

分类号： Q7

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