CHARACTERIZATION OF A NEW KUNITZ-TYPE SERINE PROTEASE INHIBITOR FROM THE HARD TICK Rhipicephalus hemaphysaloides

文献类型： 外文期刊

第一作者： Cao, Jie

作者： Cao, Jie;Shi, Lei;Zhou, Yongzhi;Gao, Xiao;Zhang, Houshuang;Gong, Haiyan;Zhou, Jinlin

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关键词： Rhipicephalus hemaphysaloides;Kunitz-type inhibitor;anticoagulant protein;Rhipilin-2

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.698； 五年影响因子：1.758 ）

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收录情况： SCI

摘要： A new Kunitz-type serine protease inhibitor, Rhipilin-2, was identified in the tick Rhipicephalus hemaphysaloides. The cDNA sequence of Rhipilin-2 is 693 bp, and it encodes a deduced 195 amino acid protein with a size of 22 kDa. Bioinformatic analysis shows that Rhipilin-2 belongs to the Kunitz-type family of inhibitors, containing one Kunitz domain with homology to the tissue factor pathway inhibitor. Using Real time polymerase chain reaction (Real time-PCR), Rhipilin-2 mRNA transcripts were detected in tick salivary glands and midgut. Blood feeding induced transcript expression. The recombinant protein was expressed in insect Sf9 cells and confirmed by immunofluorescence test and Western blot analysis with an anti-His antibody. The purified recombinant Rhipilin-2 inhibited serine protease trypsin and elastase, but not thrombin. The anticoagulant activity of Rhipilin-2 was shown by delaying normal clotting of rabbit plasma in the activated partial thromboplastin time tests. These results indicate that Rhipilin-2 is a novel Kunitz-type serine protease inhibitor involved in tick blood feeding.

分类号： Q966

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[1]Characterization of the anticoagulant protein Rhipilin-1 from the Rhipicephalus haemaphysaloides tick. Gao, Xiao,Shi, Lei,Zhou, Yongzhi,Cao, Jie,Zhang, Houshuang,Zhou, Jinlin.

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