Rice Bran Expressing a Shrimp Antimicrobial Peptide Confers Delayed Spoilage of Fish Feed and Resistance of Tilapia to Aeromonas hydrophila

文献类型： 外文期刊

第一作者： Liu, Mei

作者： Liu, Mei;Wang, Baojie;Jiang, Keyong;Gong, Kui;Sun, Shujuan;Wang, Lei;Liu, Wenzhen;Fu, Yaping

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期刊名称：JOURNAL OF THE WORLD AQUACULTURE SOCIETY （ 影响因子：2.512； 五年影响因子：2.47 ）

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收录情况： SCI

摘要： Penaeidin is an antimicrobial peptide family found in penaeid shrimp. Penaeidin3 has a broad spectrum of antimicrobial activity and is most effective against certain bacterial species. In this study, the penaeidin3-2 gene was cloned from the Chinese shrimp, Fenneropenaeus chinensis. It was then transformed into rice plants using an Agrobacterium-meAiateA transformation system. The transformants were verified by polymerase chain reaction (PCR) sequencing and real-time polymerase chain reaction (RT-PCR) analysis. To evaluate the application of penaeidin3-2 expressed by rice plants in the feedstock industry and aquaculture practice, the transgenic rice bran was added to fish feed. The proliferation of microbes in the feed was significantly inhibited bythe transgenic rice bran. Furthermore, the mortality of tilapias challenged with Aeromonas hydrophila was also significantly reduced when they were fed with the transgenic bran expressing penaeidin3-2. Their intestinal flora changed when the fish were fed with rice bran expressing the introduced gene for a relatively long period. Fish that survived the challenge had a more intact midgut structure. These results indicate that penaeidin3-2 expressed in rice plants may possess the ability to modulate theintestinal bacterial community and protect the gut structure from damage.

分类号： S96

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