Cloning and molecular characterization of phospholipase D (PLD) delta gene from longan (Dimocarpus longan Lour.)

文献类型： 外文期刊

第一作者： Zhang, Yayuan

作者： Zhang, Yayuan;Li, Li;Li, Zhichun;Li, Mingjuan;Li, Changbao;Sun, Jian;You, Xiangrong;Zhang, Yayuan;Li, Li;Li, Zhichun;Li, Mingjuan;Li, Changbao;Zhu, Jianhua;Peng, Hongxiang;Sun, Jian;Zhu, Jianhua;Peng, Hongxiang

作者机构：

关键词： Longan;Postharvest;PLD gene;Clone;Molecular characterization

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

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收录情况： SCI

摘要： Longan (Dimocarpus longan Lour.) is a non-climacteric fruit with a short postharvest life. The regulation of phospholipase D (PLD) activity closely relates to postharvest browning and senescence of longan fruit. In this study, a novel cDNA clone of longan PLD delta (LgPLD delta) was obtained and registered in GenBank (accession No. JF791814). The deduced amino acid sequence possessed all of the three typical domains of plant PLDs, a C2 domain and two catalytic HxKxxxxD motifs. The tertiary structure of LgPLD delta was further predicted. The western blot result showed that the LgPLD delta protein was specifically recognized by PLD delta antibody. The Q-RT-PCR (real-time quantitative PCR) result showed that the level of LgPLD delta mRNA expression was higher in senescent tissues than in developing tissues, which was also high in postharvest fruit. The western-blotting result further certified the different expression of LgPLD delta. These results provided a scientific basis for further investigating the mechanism of postharvest longan fruit adapting to environmental stress

分类号： Q7

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