Gene Expressing Difference in Sclerotial Formation of Morchella conica

文献类型： 外文期刊

第一作者： Chen, Li-Jiao

作者： Chen, Li-Jiao;Chen, Li-Jiao;Chai, Hong-Mei;Chen, Wei-Min;Huang, Xing-Qi;Zhao, Yong-Chang;Chai, Hong-Mei;Chen, Wei-Min;Huang, Xing-Qi;Zhao, Yong-Chang;Chai, Hong-Mei;Chen, Wei-Min;Huang, Xing-Qi;Zhao, Yong-Chang

作者机构：

关键词： mRNA differential display;Morchella conica;Sclerotia;Semi-quantitative RT-PCR

期刊名称：INDIAN JOURNAL OF MICROBIOLOGY （ 影响因子：2.461； 五年影响因子：2.259 ）

ISSN：

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收录情况： SCI

摘要： The difference of gene expression between sclerotia-producing and non-sclerotia-producing single spore isolates from Morchella conica were preliminary analyzed by mRNA differential display reverse transcription- polymerase chain reaction (RT-PCR) technique and 67 differential gene fragments were obtained. Fifty-eight of their second PCR products were cloned and sequenced. Thirteen special differential gene fragments related to sclerotial formation were validated by semi-quantitative RT-PCR. Some gene fragments had certain homologies with lipoprotein, cyclin-dependent kinase C-3, glycerophosphoryl diester phosphodiesterase, Rho GDP-dissociation inhibitor, gamma-aminobutyrate permease, OmpA family protein, Transcript antisense to ribosomal RNA protein, sodium–calcium exchange protein and keratinassociated proteins 5, 6. In addition, the putative protein of some DNA fragments had higher similarity with hypothetical protein-coding gene in NCBI database, as well as some were only putative gene fragments. All these fragments were speculated to be the functional gene associated with sclerotial formation in morel.

分类号： Q93

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