Purification and Characterization of a Thermo- and pH-Stable Laccase From the Litter-Decomposing Fungus Gymnopus luxurians and Laccase Mediator Systems for Dye Decolorization

文献类型： 外文期刊

第一作者： Sun, Yue

作者： Sun, Yue;Chen, Qing-Jun;Zhang, Guo-Qing;Sun, Yue;Liu, Zi-Lu;Hu, Bo-Yang;Yang, Ai-Zhen;Wang, Qiu-Ying;Li, Xiao-Feng;Zhang, Jia-Yan;Sun, Yue;Zhao, Yong-Chang

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关键词： Gymnopus luxurians; laccase; laccase mediator system; dye decolorization; purification

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN：

年卷期： 2021 年 12 卷

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收录情况： SCI

摘要： An extracellular laccase (GLL) was purified from fermentation broth of the litterdecomposing fungus Gymnopus luxurians by four chromatography steps, which resulted in a high specific activity of 118.82 U/mg, purification fold of 41.22, and recovery rate of 42.05%. It is a monomeric protein with a molecular weight of 64 kDa and N-terminal amino acid sequence of AIGPV TDLHI, suggesting that GLL is a typical fungal laccase. GLL demonstrated an optimum temperature range of 55 degrees C-65 degrees C and an optimum pH 2.2 toward 2,20 -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). It displayed considerably high thermostability and pH stability with about 63% activity retained after 24 h at 50 degrees C, and 86% activity retained after 24 h at pH 2.2, respectively. GLL was significantly enhanced in the presence of KC, NaC, and Mg2 C ions. It demonstrated Km of 539 mM and k(cat)/K-m of 140 mM(-1) s(-1) toward ABTS at pH 2.2 and 37 degrees C. Acetosyringone (AS) and syringaldehyde (SA) were the optimal mediators of GLL (0.4 U/ml) for dye decolorization with decolorization rates of about 60%-90% toward 11 of the 14 synthetic dyes. The optimum reaction conditions were determined to be mediator concentration of 0.1 mM, temperature range of 25 degrees C -60ffiC, and pH 4.0. The purified laccase was the first laccase isolated from genus Gymnopus with high thermostability, pH stability, and effective decolorization toward dyes, suggesting that it has potentials for textile and environmental applications.

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