Purification, characterisation and protective effects of polysaccharides from alfalfa on hepatocytes

文献类型： 外文期刊

第一作者： Wang, Shaopu

作者： Wang, Shaopu;Dong, Xiaofang;Cui, Yaoming;Tong, Jianming;Ma, Hao

作者机构：

关键词： Alfalfa polysaccharides;Monosaccharide analysis;Molecular weight;Hepatocytes;Antioxidant

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The objective of this study was to determine the preliminary characteristics and protective effects of alfalfa polysaccharides (APS) on hepatocytes in vitro. The crude APS was purified by DEAE-cellulose and Sephadex G-l 00 chromatography, resulting in the four purified fractions: APS-1, APS-2, APS-3 and APS-4. The results indicated that APS-3 had higher carbohydrate and uronic acid contents and that APS-4 had a more complicated monosaccharide composition compared to the other purified fractions. The average molecular weights of APS-1, APS-2, APS-3 and APS-4 were 48,536,6,221,66,559 and 13,076 Da, respectively. Furthermore, APS (crude and its purified fractions) restored the activities of antioxidant enzymes and increased the total antioxidant capacity of hepatocytes subjected to H2O2-induced oxidative stress. Furthermore, APS treatment counteracted the increases in lactic dehydrogenase and malonaldehyde in the culture supernatant. These results clearly demonstrate that APS possesses a protective effect against oxidative injury in hepatocytes.

分类号： O629`Q53

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