Three in one: Identification, expression and enzymatic activity of lysozymes in amphioxus

文献类型： 外文期刊

第一作者： Xu, Na

作者： Xu, Na;Xu, Na;Pan, Junli;Liu, Shousheng;Zhang, Shicui;Xu, Na;Pan, Junli;Liu, Shousheng;Zhang, Shicui;Xue, Qinggang

作者机构：

关键词： Protochordate;Amphioxus;G-type lysozyme;C-type lysozyme;I-type lysozyme

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

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年卷期：

页码：

收录情况： SCI

摘要： The lysozymes identified so far in animals belong to the g-type, c-type, and i-type. Vertebrate animals possess only the former two types, i.e., g- and c-types, while all the three types have been reported in invertebrates. Here we demonstrate that (1) three cDNAs that encode g-, c-, and i-type lysozymes, respectively, were identified in a single species of the amphioxus Branchiostoma japonicum; (2) all the 3-type genes displayed distinct tissue-specific expression pattern; (3) recombinant g-, c-, and i-type lysozymes all exhibited enzymatic activities; and (4) native g-, c-, and i-type lysozymes were identified in the different tissues of amphioxus. Collectively, these results suggest the presence of all the 3-type lysozymes in a single animal species, first such data ever reported. The presence of biologically active i-type lysozyme in amphioxus also suggests that i-type lysozyme gene is retained at least in Protochordata, contrasting to the previous proposal that i-type lysozyme gene has been lost in a common ancestor of all chordates.

分类号： R392

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