Identification of 23 novel conserved microRNAs in three rice cultivars

文献类型： 外文期刊

第一作者： Yang, Jian

作者： Yang, Jian;Zhang, Heng-Mu;Liu, Xiao-Ya;Li, Jing;Lv, Ming-Fang;Li, Pei-Pei;Chen, Jian-Ping;Liu, Xiao-Ya;Li, Pei-Pei;Dai, Liang-Ying

作者机构：

关键词： High-throughput RNA sequencing;MicroRNAs (miRNAs);Quantitative real-time polymerase chain reaction (qPCR);Rice (Oryza sativa)

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Plant microRNAs (miRNAs) play important roles as modulators of gene expression at the post-transcriptional level. Previous studies have shown that high-throughput sequencing is a powerful tool for the identification of miRNAs, and it is believed that many more miRNAs remain to be discovered. Here, we found 23 novel conserved miRNAs from three rice cultivars by high-throughput sequencing and further identified these through subsequent cloning and quantitative real-time polymerase chain reaction (qPCR). Eight of these novel miRNAs were detected with significant signals in the three rice cultivars by northern blotting assays. The quantitative analysis of their expression profiles showed that most of these miRNAs were perfectly or imperfectly negatively correlated with their target genes, which suggests that these miRNAs may play important roles during rice development. This is the first genome-wide investigation of miRNAs from different rice cultivars, and the data obtained expand the known rice miRNA inventory and provide further information about the regulatory roles played by miRNAs in rice development.

分类号： R394

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