Isolation and characterization of mesenchymal stem cells derived from fetal bovine liver

文献类型： 外文期刊

第一作者： Lu, Taofeng

作者： Lu, Taofeng;Hu, Pengfei;Su, Xiaohua;Li, Changli;Ma, Yuehui;Guan, Weijun

作者机构：

关键词： Bovine;LMSCs;Proliferation;Multi-lineage differentiation

期刊名称：CELL AND TISSUE BANKING （ 影响因子：1.522； 五年影响因子：1.968 ）

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年卷期：

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收录情况： SCI

摘要： Bovine liver-derived mesenchymal stem cells (bLMSCs) were isolated from the liver tissue of 4–6 months old fetal calf, and then characterized by immunofluorescence and RT-PCR. We found that primary bLMSCs could be subcultured to 44 passages, the total culture time in vitro was 192 days. The results of surface antigen detection showed that bBMSCs expressed CD29, CD44, CD73, CD90, CD106 and CD166 but not expressed endothelial cells and hematopoietic cells specific marker CD34, CD45 and BLADR. The results of growth kinetics, colony-forming cell assay and cell cycle analysis indicated that the fetal bovine LMSCs had good proliferation ability in vitro. The cells from passages 7 were successfully induced to differentiate into osteoblasts, adipocytes and chondrocytes. The results indicate the potential for multi-lineage differentiation of bLMSCs that may represent an ideal candidate for cellular transplantation therapy.

分类号： Q2

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