Biodegradation of neonicotinoid insecticide, imidacloprid by restriction enzyme mediated integration (REMI) generated Trichoderma mutants
文献类型: 外文期刊
第一作者: He, Xiangfeng
作者: He, Xiangfeng;Wubie, Abebe Jenberie;Diao, Qingyun;Li, Wei;Xue, Fei;Guo, Zhanbo;Zhou, Ting;Xu, Shufa;Wubie, Abebe Jenberie
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关键词: REMI;Trichoderma;Neonicotinoid insecticide;Imidacloprid;Biodegradation
期刊名称:CHEMOSPHERE ( 影响因子:7.086; 五年影响因子:6.956 )
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收录情况: SCI
摘要: REMI (restriction enzyme-mediated integration) technique was employed to construct Trichoderma atroviride strain T23 mutants with degrading capability of neonicotinoid insecticide, imidacloprid. The plasmid pBluescript Ⅱ KS-hph used for integration in REMI mutants was confirmed by PCR and Southern hybridization. Among 153 transformants, 57% of them have showed higher neonicotinoid insecticide, imidacloprid, degradation ability than the wild strain T23 (p < 0.01). More specifically, seven single-copied T. atroviride T23 transformants have confirmed a 30% higher degradation rate than their parent isolate. Among all transformed mutants, a 95% imidacloprid degradation rate was identified as the highest. This study, thus, provided an effective approach for improving neonicotinoid insecticide-degrading capability using REMI transformed T. atroviride mutants.
分类号: X5
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