Odorant Binding Characteristics of Three Recombinant Odorant Binding Proteins in Microplitis mediator (Hymenoptera: Braconidae)

文献类型： 外文期刊

第一作者： Wang, Shanning

作者： Wang, Shanning;Ren, Liyan;Ali, Abid;Zhang, Yongjun;Guo, Yuyuan;Li, Keming;Zhang, Kang;Zhou, Jingjiang

作者机构：

关键词： Microplitis mediator;Odorant binding protein;Tissue distribution;Fluorescence competitive binding assay;Binding characteristic;Y-tube assay

期刊名称：JOURNAL OF CHEMICAL ECOLOGY （ 影响因子：2.626； 五年影响因子：2.984 ）

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收录情况： SCI

摘要： Odorant binding proteins (OBPs) are believed to be important for transporting semiochemicals through the aqueous sensillar lymph to the olfactory receptor cells within the insect antennal sensilla. In this study, three new putative OBP genes, MmedOBP8-10, were identified from a Microplitis mediator (Hymenoptera: Braconidae) antennal cDNA library. Quantitative real-time PCR (qRT-PCR) analysis revealed that all three of the OBP genes were expressed mainly in the antennae of adult wasps. The three OBPs were recombinantly expressed in Escherichia coli and purified by Ni ion affinity chromatography. Fluorescence competitive binding assays were performed using N-phenyl-naphthylamine as a fluorescent probe and 45 small organic compounds as competitors. These assays demonstrated that the three M. mediator OBPs can bind a broad range of odorant molecules with different binding affinities. They can bind the following ligands: nonane, farnesol, nerolidol, nonanal, beta-ionone, acetic ether, and farnesene. In a Y-tube assay with these ligands as odor stimuli and paraffin oil as a control, all ligands, except nerolidol and acetic ether, were able to elicit behavioral responses in adult M. mediator. The wasps were significantly attracted to beta-ionone, nonanal, and farnesene and repelled by nonane and farnesol. The results of this work provide insight into the chemosensory functions of the OBPs in M. mediator.

分类号： Q14

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