Screening of high toxic Metarhizium strain against Plutella xylostella and its marking with green fluorescent protein

文献类型： 外文期刊

第一作者： Cui, Qianqian

作者： Cui, Qianqian;Zhang, Yi;Zang, Yanchao;Nong, Xiangqun;Wang, Guangjun;Zhang, Zehua

作者机构：

关键词： Metarhizium anisopliae;Plutella xylostella;Green fluorescent protein (GFP)

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Entomopathogenic fungus is proposed to be one of the best biocontrol agents against the destructive insect pest Plutella xylostella. In this study, we tested the virulence of 11 Metarhizium strain isolates against P. xylostella using a leaf dipping method, and found one strain, named 609, which had displayed the highest pathogenicity. Bioassay results showed that the accumulated corrected mortality rate was 86.7 % on the eighth day after inoculation with a spore concentration 1 x 10(8) conidia/mL, and that the time to 50 % lethality was 5.7-day. The strain was identified as Metarhizium anisopliae var. acridum by internal transcribed spacer (ITS) region sequencing. A green fluorescent protein (GFP) marker containing vector, camben-gfp, was constructed and delivered into strain 609 by Agrobacterium tumefaciens-mediated transformation. Six positive clones expressing GFP were selected and tested for toxicity against P. xylostella, all of which displayed the same toxicity as the parental wild type strain. The survival rate of transformant T1 was investigated by monitoring GFP levels at 4-day intervals after inoculation into soil. We found that the concentration of Metarhizium spores decreased sharply from 1 x 10(7) conidia/g to 1 x 10(6) conidia/g in the first 5 days after inoculation. The decreasing trend then stabilized and the spore count declined to approximately 1 x 10(4)-10(5) conidia/g after 1 month. The results of this study indicate that the expression of gfp gene in strain 609 does not alter the virulence capability of Metarhizium. This strain will therefore be useful for the control of P. xylostella and as a tool to study molecular biology properties and monitor colonization of M. anisopliae in the field

分类号： Q93

  • 相关文献

[1]Colonization of Fusarium Wilt-Resistant and Susceptible Watermelon Roots by a Green-Fluorescent-Protein-tagged Isolate of Fusarium oxysporum f.sp.niveum. Lu, Guiyun,Guo, Shaogui,Zhang, Haiying,Geng, Lihua,Xu, Yong,Lu, Guiyun,Martyn, Raymond D.. 2014

[2]Jellyfish Green Fluorescent Protein (GFP) as a Reporter for Fusarium gramminearum Development on Wheat. Liu Tai-guo,Xu Ying,Gao Li,Liu Bo,Chen Wan-quan,Chen Huai-gu. 2014

[3]Bacillus amyloliquefaciens GB1 can effectively control apple valsa canker. Zhang, Jun-xiang,Gu, Yan-bing,Chi, Fu-mei,Ji, Zhi-rui,Wu, Jian-yuan,Dong, Qing-long,Zhou, Zong-shan.

[4]Entomopathogenicity of Three Muscardine Fungi, Beauveria bassiana, Isaria fumosorosea and Metarhizium anisopliae, against the Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae). Ma, Chun-Sen,Majeed, M. Z.,Fiaz, M.,Afzal, M.. 2017

[5]Screening of tropical isolates of Metarhizium anisopliae for virulence to the red palm weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae). Sun, Xiaodong,Yan, Wei,Qin, Weiquan,Zhang, Jing,Niu, Xiaoqing,Sun, Xiaodong,Li, Fuheng,Ma, Guangchang. 2016

[6]Nutritional requirements of mycelial growth and sporulation of several biocontrol fungi in submerged and on solid culture. Liu, Xingzhong,Gao, Li.

[7]Phoxim and manure enhanced the population rebound and preservation of Metarhizium anisopliae applied in soil. Nong, Xiangqun,Liu, Xun,Dong, Changzhong,Wang, Guangjun,Cao, Guangchun,Zhang, Zehua,Wang, Qinglei.

[8]Persistence and proliferation of a Chinese Metarhizium anisopliae s.s. isolate in the peanut plant root zone. Nong, Xiangqun,Li, Xingjia,Wang, Guangjun,Cao, Guangchun,Zhang, Zehua,Wang, Qinglei.

[9]Laboratory evaluation of virulence of Chinese Beauveria bassiana and Metarhizium anisopliae isolates to engorged female Rhipicephalus (Boophilus) microplus ticks. Ren, Qiaoyun,Liu, Zhijie,Guan, Guiquan,Sun, Ming,Ma, Miling,Niu, Qingli,Li, Youquan,Liu, Aihong,Liu, Junlong,Yang, Jifei,Yin, Hong,Luo, Jianxun. 2012

[10]Virulence of Beauveria bassiana, Metarhizium anisopliae and Paecilomyces lilacinus to the engorged female Hyalomma anatolicum anatolicum tick (Acari: Ixodidae). Sun, Ming,Ren, Qiaoyun,Guan, Guiquan,Liu, Zhijie,Ma, Miling,Gou, Huitian,Chen, Ze,Li, Youquan,Liu, Aihong,Niu, Qingli,Yang, Jifei,Yin, Hong,Luo, Jianxun. 2011

[11]Susceptibility of the tick Haemaphysalis qinghaiensis to isolates of the fungus Metarhizium anisopliae in China. Ren, Qiaoyun,Sun, Ming,Guan, Guiquan,Liu, Zhijie,Chen, Ze,Liu, Aihong,Li, Youquan,Ma, Miling,Yang, Jifei,Niu, Qingli,Liu, Junlong,Yin, Hong,Luo, Jianxun,Han, Xueqing.

[12]Laboratory evaluation of Beauveria bassiana and Metarhizium anisopliae in the control of Haemaphysalis qinghaiensis in China. Ren, Qiaoyun,Chen, Ze,Luo, Jin,Liu, Guangyuan,Guan, Guiquan,Liu, Zhijie,Liu, Aihong,Li, Youquan,Niu, Qingli,Liu, Junlong,Yang, Jifei,Yin, Hong,Luo, Jianxun,Yin, Hong,Han, Xueqing.

[13]Frozen section and electron microscopy studies of the infection of the red palm weevil, Rhynchophorus ferrugineus (coleoptera: curculionidae) by the entomopathogenic fungus Metarhizium anisopliae. Sun, Xiaodong,Yan, Wei,Zhang, Jing,Niu, Xiaoqing,Qin, Weiquan,Sun, Xiaodong,Li, Fuheng,Ma, Guangchang. 2016

[14]A novel two-stage cultivation method to optimize carbon concentration and carbon-to-nitrogen ratio for sporulation of biocontrol fungi. Liu, X. Z.,Gao, L..

[15]cDNA cloning and characterization of the carboxylesterase pxCCE016b from the diamondback moth, Plutella xylostella L.. Hu Zhen-di,Liang Pei,Gao Xi-wu,Hu Zhen-di,Feng Xia,Lin Qing-sheng,Chen Huan-yu,Li Zhen-yu,Yin Fei. 2016

[16]A comparison of the performance and biological efficiency of new knapsack sprayers and a controlled droplet application (CDA) sprayer for the control of the diamondback moth, Plutella xylostella L. (Lepidoptera : Plutellidae). Guo, SJ,Geitz, G,Franklin, T,Liu, YQ,Furlong, MJ,Zalucki, MP,Cooper, L. 2004

[17]Pest control and resistance management through release of insects carrying a male-selecting transgene. Harvey-Samuel, Tim,Alphey, Nina,Alphey, Luke,Harvey-Samuel, Tim,Morrison, Neil I.,Walker, Adam S.,Marubbi, Thea,Gorman, Kevin,Warner, Simon,Alphey, Luke,Yao, Ju,Collins, Hilda L.,Shelton, Anthony M.,Yao, Ju,Davies, T. G. Emyr,Alphey, Nina,Alphey, Luke. 2015

[18]Cloning, ligand-binding, and temporal expression of ecdysteroid receptors in the diamondback moth, Plutella xylostella. Tang, Baozhen,Dong, Wei,Liang, Pei,Gao, Xiwu,Dong, Wei,Zhou, Xuguo. 2012

[19]Joint Application of Isaria fumosorsea Chitinase and Azadirachtin as Biopesticides against the Diamondback Moth, Plutella xylostella L. (Lepidoptera: Plutellidae). Long, X. Z.,Yu, Y. H.,Huang, T. T.,Ali, S.. 2016

[20]Different genetic structures revealed resident populations of a specialist parasitoid wasp in contrast to its migratory host. Wei, Shu-Jun,Zhou, Yuan,Fan, Xu-Lei,Cao, Li-Jun,Zhou, Yuan,Xu, Zai-Fu,Hoffmann, Ary A.,Chen, Xue-Xin. 2017

作者其他论文 更多>>

微信小程序