All-trans Retinoic Acid Promotes Nerve Cell Differentiation of Yolk Sac-Derived Mesenchymal Stem Cells

文献类型： 外文期刊

第一作者： Gao, Yuhua

作者： Gao, Yuhua;Wang, Kunfu;Zheng, Dong;Gao, Yuhua;Bai, Chunyu;Wang, Kunfu;Sun, Bo;Guan, Weijun

作者机构：

关键词： Differentiation;All-trans retinoic acid;Nerve;Chick

期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）

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年卷期：

页码：

收录情况： SCI

摘要： Fetal membranes are abundant; the yolk sac is a source of cell lineages that do not express MHCs and are mainly free from immunological incompatibles when transferred to a recipient. Although data are available especially for hematopoietic stem cells in human and murine; whereas other cell types and species are dramatically unnoticed. Here, we studied the nature and differentiation potential of yolk sac-derived mesenchymal stem cells from a chicken embryo. In this study, we observed the gene expression of pluripotent markers in yolk sac mesenchymal stem cells (YS-MSCs) and the capacity of YS-MSCs to differentiate into neurallike cells using quantitative RT-PCR, immunocytochemistry, and western blotting. YS-MSCs have a spindle shape and revealed the expression of the MSC-related proteins β-integrin, CD44, CD71, and CD73, but not CD34. YS-MSCs express pluripotent markers such as octamer-binding transcription factor 4 (Oct4) and Nanog at the protein and mRNA levels. QRT-PCR analyses revealed that YS-MSCs expressed nestin. Immunocytochemical and western blotting data showed that the cells expressed Nestin and microtubule-associated protein 2 (Map-2) for neurons, respectively, after induction of neural differentiation. These findings demonstrate the plasticity of YS-MSCs and their potential for use in cellular replacement therapy for neural diseases.

分类号： Q5

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