Integrative analysis of transcriptomic and metabolomic profiling of ascites syndrome in broiler chickens induced by low temperature

文献类型: 外文期刊

第一作者: Shi, Shourong

作者: Shi, Shourong;Hou, Zhuocheng;Yang, Ying;Guo, Yuming;Shi, Shourong;Shen, Yiru;Zhao, Zhenhua;Zou, Jianmin;Zhao, Zhenhua;Zhou, Huaijun;Zou, Jianmin

作者机构:

关键词: Integrative;analysis;transcriptomic

期刊名称:MOLECULAR BIOSYSTEMS ( 影响因子:3.336; 五年影响因子:2.986 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Ascites syndrome (AS) still has an unacceptably high incidence rate in both humans and animals although there have been many studies on AS. To continue our previous pathological and biochemical investigation on the underlying mechanisms of AS incidence in broiler chickens, cutting-edge technologies including RNA-seq and metabolimics were used by directly comparing AS chickens and healthy controls. The RNA-seq analysis in the liver identified 390 differentially expressed genes (DEGs), among which 212 genes were up-regulated and 178 genes were down-regulated in the AS group compared to the control. For the downregulated DEGs, further gene ontology (GO) analysis suggested that lipid metabolism, cell differentiation, enzyme linked receptor protein signaling pathway and steroid biosynthesis pathway were significantly enriched. For up-regulated DEGs, the cholesterol metabolic process has the lowest p value (0.000966) of fold enrichment while the cholesterol biosynthetic process has the highest fold enrichment (46.67). The metabolomic analysis of serum revealed statistically significant changes in the concentrations of LysoPC(20:4), LysoPC(16: 0), LysoPC(18:0), LysoPC(18:1), LysoPC(18:2), PC(14:1/20:1), PC(20:4/18:0), PC(14:1/22:1), dihydroxyacetone, indoleacrylic acid, ursodeoxycholic acid, L-valine, and L-tryptophan. The integrative analysis of transcriptome and metabolome indicated that two biological pathways of tryptophan biosynthesis and metabolism, and glycerophospholipid metabolism may contribute to the induction of AS in broilers. These findings have provided novel insights into our understanding of molecular mechanisms of AS incidence in broilers.

分类号: Q7`Q5

  • 相关文献

[1]Physiological and Transcriptomic Responses of Chinese Cabbage (Brassica rapa L. ssp Pekinensis) to Salt Stress. Qiu, Nianwei,Li, Jingjuan,Zhang, Yihui,Wang, Fengde,Gao, Jianwei,Qiu, Nianwei,Liu, Qian. 2017

[2]Comparative transcriptomic analysis of resistant and susceptible alfalfa cultivars (Medicago sativa L.) after thrips infestation. Zhang, Zehua,Liu, Zhongkuan. 2018

[3]Transcriptome assembly and expression profiling of the molecular responses to cadmium toxicity in cerebral ganglia of wolf spider Pardosa pseudoannulata (Araneae: Lycosidae). Yang, Huilin,Yang, Huilin,Shi, Yixue,Wang, Juan,Wang, Zhi,Peng, Yuande,Xie, Chunliang,Tian, Jianxiang,Peng, Xianjin,Xu, Xiang,Song, Qisheng,Lv, Zhiyue. 2018

[4]Ionomic and transcriptomic analysis provides new insight into the distribution and transport of cadmium and arsenic in rice. Feng, Xuemin,Han, Lei,Liu, Yan,Zhang, Yajing,Wang, Ruigang,Guo, Junkang,Feng, Renwei,Xu, Yingming,Ding, Yongzhen,Zhang, Guilong,Feng, Xuemin,Han, Lei,Liu, Yan,Zhang, Yajing,Wang, Ruigang,Guo, Junkang,Feng, Renwei,Xu, Yingming,Ding, Yongzhen,Zhang, Guilong,Chao, Daiyin,Huang, Biyan.

[5]Determination of free steroidal compounds in vegetable oils by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry. Xu, Baocheng,Zhang, Liangxiao,Ma, Fei,Zhang, Wen,Wang, Xiupin,Zhang, Qi,Li, Peiwu,Xu, Baocheng,Luo, Denglin,Ma, Hongyan,Zhang, Qi,Zhang, Qi,Li, Peiwu,Zhang, Liangxiao,Ma, Fei,Zhang, Wen,Wang, Xiupin,Li, Peiwu,Zhang, Liangxiao,Ma, Fei,Zhang, Wen,Wang, Xiupin,Li, Peiwu. 2018

[6]Morphological comparison between a selected fast-growing strain and the common cultured strain of turbot Scophthalmus maximus. Liang Xingming,Ma Aijun,Wang Xin'an,Li Juan,Huang Zhihui,Shang Xiaomei. 2012

[7]Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens. Wu, Jian,Ghandilyan, Artak,Guzman, Myriam Olortegui,Aarts, Mark G. M.,Wu, Jian,Wang, Xiaowu,Zhao, Fang-Jie,Logoteta, Barbara,Schat, Henk.

[8]Complete DNA sequence and gene analysis of the virulence plasmid pCP301 of Shigella flexneri 2a. Zhang, JY,Liu, H,Zhang, XB,Yang, J,Yang, F,Yang, GW,Shen, Y,Hou, YD,Jin, Q.

[9]Complete Genome Sequence of Goose Parvovirus Isolated from Anser cygnoides in China. Wan, Chun-he,Hu, Kai-hui,Wan, Chun-he,Huang, Yu,Shi, Shao-hua,Fu, Guang-hua,Cheng, Long-fei,Chen, Hong-mei,Fu, Qiu-ling,Chen, Cui-teng. 2016

[10]Fusion of C3d with hemagglutinin enhances protective immunity against swine influenza virus. Li, Guo-Xin,Tian, Zhi-Jun,Yu, Hai,Jin, Yuan-Yuan,Hou, Shao-Hua,Zhou, Yan-Jun,Liu, Tian-Qiang,Hu, Shou-Ping,Tong, Guang-Zhi,Li, Guo-Xin,Tong, Guang-Zhi,Li, Guo-Xin,Yu, Hai,Zhou, Yan-Jun,Tong, Guang-Zhi.

[11]Analysis of Income Difference among Rural Residents in China. Xue, Yan,Zhu, Yeping,Li, Shijuan. 2011

[12]Investigation of N and P Non-point Source Pollution Status of Vegetable Fields in the Suburb of Fuzhou City. Huang Dong-feng,Qiu Xiao-xuan,Li Wei-hua,Fan Ping. 2010

[13]Molecular Cloning, Characterization, and Expression of MiSOC1: A Homolog of the Flowering Gene SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 from Mango (Mangifera indica L). Wei, Junya,Liu, Debing,Liu, Guoyin,Tang, Jie,Wei, Junya,Chen, Yeyuan. 2016

[14]Analytical approach, dissipation pattern and risk assessment of pesticide residue in green leafy vegetables: A comprehensive review. Farha, Waziha,Rahman, Md Musfiqur,Shim, Jae-Han,Abd El-Aty, A. M.,Abd El-Aty, A. M.,Shin, Ho-Chul,Jeong, Ji Hoon,Wang, Jing,Shin, Sung Shik. 2018

[15]Analysis of N-terminal nucleotide sequence of NDV fusion protein. Wang, WB,Ji, P,He, JL,Wu, XF. 1999

[16]Visual assessment of soil structure: Evaluation of methodologies on sites in Canada, China and Germany Part I: Comparing visual methods and linking them with soil physical data and grain yield of cereals. Mueller, Lothar,Schindler, Uwe,Kay, Bev D.,Hu, Chunsheng,Li, Yong,Behrendt, Axel,Shepherd, T. Graham,Ball, Bruce C.. 2009

[17]Maize and soybean comparative advantage analysis of counties in Jilin province. Xue, Yan,Zhu, Yeping,Li, Shijuan. 2007

[18]MOLECULAR CLONING AND ANALYSIS OF A CONSTANS HOMOLOG FROM NICOTIANA TABACUM. Lu, Y.,Liu, Y.,Sun, Y.,Mu, J.,Ren, M.,Zhang, X.,Wang, Z.. 2013

[19]Dynamics of soil carbon to nitrogen ratio changes under long-term fertilizer addition in wheat-corn double cropping systems of China. Wang, X. J.,Cong, R. H.,Xu, M. G.,Zhang, W. J.,Xie, L. J.,Wang, B. R.,Cong, R. H.,Wang, X. J.,Huang, S. M..

[20]Simultaneous targeted analysis of five active compounds in licorice by ultra-fast liquid chromatography coupled to hybrid linear-ion trap tandem mass spectrometry. Kong, Weijun,Wen, Jing,Yang, Yinhui,Qiu, Feng,Yang, Meihua,Wen, Jing,Yang, Yinhui,Sheng, Ping.

作者其他论文 更多>>