Molecular cloning and characterization of two alanine aminotransferase genes in the white-backed planthopper Sogatella furcifera

文献类型： 外文期刊

第一作者： Fan, Jin-Mei

作者： Fan, Jin-Mei;Wan, Pin-Jun;Li, Na;Mu, Li-Li;Li, Guo-Qing;Fu, Qiang

作者机构：

关键词： Sogatella furcifera;Alanine aminotransferase;Recombinant proteins;Enzyme activity

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）

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收录情况： SCI

摘要： Sogatella furcifera is a serious phloem-feeding pest of rice. Rice phloem sap cannot meet its nutrition requirement for some essential amino acids. The yeast-like symbionts (YLSs) have been hypothesized to aid S. furcifera in amino acid metabolism to compensate for the unbalanced amino acid composition in the rice phloem. In the present paper, two full-length cDNAs of putative alanine aminotransferases (ALTs) were obtained from S. furcifera. Sequence similarity and phylogenetic analysis suggested that one ALT came from the YLSs (SfylsALT) whereas the other ALT originated from S. furcifera (SfALT1). Quantitative reverse transcription polymerase chain reaction revealed that SfylsALT clearly had a high transcript level in the abdomen. In contrast, SfALT1 was broadly expressed in the head, wings, legs, thorax and abdomen. Since YLSs were distributed in the abdominal fat body, ovary and eggs, and were not located in the head, leg, thorax and wing, the tissue expression patterns also suggested that SfylsALT came from the YLSs' genome and SfALT1 was from the host. Moreover, the enzyme activities of Sf9 cell-expressed SfALT1 and SfylsALT proteins were tested in vitro. The isolated suspensions containing either of the two proteins showed higher ALT activities than that from blank cells or egfp-expressed cells. It seems that SfALT1 and SfylsALT encode functional alanine aminotransferases

分类号： Q96

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