Identification and Characterization of Genes Differentially Expressed in X and Y Sperm Using Suppression Subtractive Hybridization and cDNA Microarray

文献类型： 外文期刊

第一作者： Chen, Xiaoli

作者： Chen, Xiaoli;Yue, Yang;He, Yanan;Zhu, Huabin;Hao, Haisheng;Zhao, Xueming;Qin, Tong;Wang, Dong

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期刊名称：MOLECULAR REPRODUCTION AND DEVELOPMENT （ 影响因子：2.609； 五年影响因子：3.47 ）

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收录情况： SCI

摘要： Differential expression of genes leads to variations in the phenotypes of X and Y sperm, although some differentially expressed gene products are shared through intercellular bridges. Genes differentially expressed in bovine X and Y sperm were identified by a combination of suppression subtractive hybridization (SSH), cDNA microarray, and sequence-homology analysis. Microarray data and Significance Analysis of Microarrays software were used to identify 31 differentially expressed genes, only four of which were previously identified. These genes are involved in fundamental life processes of mature sperm, and may be associated with the differences between X and Y sperm since 27 versus 4 were upregulated in X versus Y sperm, respectively. The levels of expression of seven genesincluding the known genes UTY, DPH3, CYTB, and ISCU, and the unknown genes X+Y contig 41, X+Y contig 18, and Y+X contig 16were validated by quantitative real-time PCR, and some genes were clearly differentially expressed by X and Y sperm, despite the presence of intercellular bridges among spermatids. These results provide a theoretical basis for research on gene expression during sperm development, as well as on sex control at the level of sperm. Mol. Reprod. Dev. 81: 908-917, 2014

分类号： Q7

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