Detection of Nosema bombycis by FTA Cards and Loop-Mediated Isothermal Amplification (LAMP)

文献类型： 外文期刊

第一作者： Yan, Wei

作者： Yan, Wei;Shen, Zhongyuan;Tang, Xudong;Xu, Li;Li, Qianlong;Yue, Yajie;Xiao, Shengyan;Fu, Xuliang;Shen, Zhongyuan;Tang, Xudong;Xu, Li;Li, Qianlong;Yue, Yajie;Xiao, Shengyan;Shen, Zhongyuan

作者机构：

期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： We successfully established a detection method which exhibited a markedly higher sensitivity than previously developed detection methods for Nosema bombycis by combining glass beads, FTA card, and LAMP. Spores of N. bombycis were first broken by acid-washed glass beads; the DNA was subsequently extracted and purified with the FTA card, and LAMP was performed using primers (LSU296) designed based on the sequence of the LSU rRNA of N. bombycis. The minimum detection concentration was 10 spores/mL. When this method was used to detect pebrine disease in silkworm egg, the detection rate for 500 silkworm eggs, in which only one egg was infected with N. bombycis, was 100 % under our optimized conditions. If the number of eggs in the sample increased to 800 or 1,000, the sample was divided into two equal portions, and the eggs were smashed with glass beads after the addition of 1 mL of TE buffer. The liquid in two tubes was later mixed and applied to the FTA card, and the detection rates were 100 %. Furthermore, the LAMP method established in our study could detect N. bombycis infection in silkworm 24 h earlier than microscopy.

分类号： Q93

  • 相关文献
作者其他论文 更多>>

微信小程序