Functional characterization of sex pheromone receptors in the purple stem borer, Sesamia inferens (Walker)

文献类型： 外文期刊

第一作者： Zhang, J.

作者： Zhang, J.;Yan, S. -W.;Chang, H. -T.;Dong, S. -L.;Zhang, J.;Yan, S. -W.;Chang, H. -T.;Liu, Y.;Wang, G. -R.;Zhang, Y. -N.

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关键词： purple stem borer;pheromone receptor;Z-11-hexadecenyl acetate (Z11-16:OAc);Z-11-hexadecenol (Z11-16:OH);Xenopus oocytes

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）

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收录情况： SCI

摘要： The sex pheromone communication system in moths is highly species-specific and extremely sensitive, and pheromone receptors (PRs) are thought to be the most important factors in males. In the present study, three full-length cDNAs encoding PRs were characterized from Sesamia inferens antennae. These three PRs were all male-specific in expression, but their relative expression levels were very different; SinfOR29 was 17-to 23-fold higher than the other two PRs. Phylogenetic and motif pattern analyses showed that these three PRs were allocated to different PR subfamilies with different motif patterns. Functional analysis using the heterologous expression system of Xenopus oocytes demonstrated that SinfOR29 specifically and sensitively responded to the major pheromone component, Z11-16:OAc [concentration for 50% of maximal effect (EC50) = 3.431 x 10(-7) M], while SinfOR21 responded robustly to a minor pheromone component Z11-16: OH (EC50 = 1.087 x 10(-6) M). SinfOR27, however, displayed no response to any of the three pheromone components, but, interestingly, it was sensitive to a non-sex pheromone component Z9, E12-14: OAc (EC50 = 1.522 x 10(-6) M). Our results provide insight into the molecular mechanisms of specificity and sensitivity of the sex pheromone communication system in moths.

分类号： R47

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