A novel, in vivo, indoor method to preserve rice black-streaked dwarf virus in small brown planthopper using wheat seedling as a bridge host

文献类型： 外文期刊

第一作者： Ren, Chunmei

作者： Ren, Chunmei;Cheng, Zhaobang;Yang, Liu;Miao, Qian;Fan, Yongjian;Zhou, Yijun

作者机构：

关键词： Rice black-streaked dwarf virus;Wheat;Rice;Small brown planthopper (Laodelphgax striatellus);In vivo preservation

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Rice black-streaked dwarf virus (RBSDV) naturally infects Gramineae plants through small brown planthopper (SBPH) as a vector. However, RBSDV cannot be transmitted to the SBPH offspring through transovarian transmission. Wheat plant, an important intermediate host in winter, is essential for the completion of the annual cycle of RBSDV in farm ecosystem. We developed a novel, in vivo, indoor method to preserve RBSDV in SBPH using wheat seedling as a bridge host. The temperature range of 23-27 degrees C was initially selected to rear the insects and plants. Before initiating the scheme cycle, viruliferous SBPH was obtained by feeding the virus-free 1st to 2nd instar nymphs with RBSDV-infected rice plants. Four to six RBSDV-infected SBPH were placed per plant to inoculate wheat seedlings at two-to-four leaf stages. After 48 h of inoculation, the viruliferous SBPH were removed. Five mated, newly emerged virus-free SBPH females were then transferred onto each inoculated plant and allowed to lay eggs for 48 h. The newly hatched SBPH were raised on wheat seedlings until the 2nd instar nymph stage, and then transferred onto healthy rice seedlings for further development until 5th instar nymphs or adults. These newly obtained viruliferous SBPH can be used for inoculating new wheat seedlings in the succeeding maintenance cycles, or for further experiments. We discovered that the incubation period of RBSDV in wheat seedlings synchronized with the gestation period of SBPH eggs at four to six inoculated viruliferous SBPH per plant and lasted for approximately seven days. In addition, this period was optimal for enhancing the SBPH infection ratio because SBPH nymphs can only acquire the virus after they hatch. The RBSDV infection ratio of the SBPHs acquired through this method consistently exceeded 50%

分类号： R37

  • 相关文献

[1]Distribution Characteristics of Soil Cadmium in Different Textured Paddy Soil Profiles and Its Relevance with Cadmium Uptake by Crops. Wang Zheng-yin,Qin Yu-sheng,Zhan Shao-jun,Yu Hua,Tu Shi-hua. 2013

[2]The effect of low water content on seed longevity. Hu, CL,Zhang, YL,Tao, M,Hu, XR,Jiang, CY. 1998

[3]Phosphorus Efficacy in Four Chinese Long-Term Experiments with Different Soil Properties and Climate Characteristics. Zhao, Linping,Ma, Yibing,Liang, Guoqing,Li, Shutian,Wu, Lishu,Zhao, Linping,Ma, Yibing,Liang, Guoqing,Li, Shutian,Zhao, Linping,Wu, Lishu. 2009

[4]Homologous analysis of SSR-ESTs and transferability of wheat SSR-EST markers across barley, rice and maize. Tang, Jifeng,Gao, Lifeng,Cao, Yongsheng,Jia, Jizeng. 2006

[5]Dynamics of the evolution of orthologous and paralogous portions of a complex locus region in two genomes of allopolyploid wheat. Kong, XY,Gu, YQ,You, FM,Dubcovsky, J,Anderson, OD.

[6]Inorganic fertilizer application ensures high crop yields in modern agriculture: A large-scale field case study in Central China. Wang, Weini,Lu, Jianwei,Ren, Tao,Li, Yinshui,Zou, Juan,Su, Wei,Li, Xiaokun,Li, Yinshui,Zou, Juan. 2012

[7]Soil microbial biomass, crop yields, and bacterial community structure as affected by long-term fertilizer treatments under wheat-rice cropping. Zhang, Xiaoping,Tu, Shihua,Lindstrom, Kristina. 2009

[8]Starch structure and digestibility of rice high in resistant starch. Shu, Xiaoli,Jiao, Guiai,Fitzgerald, Melissa A.,Yang, Chaozhu,Shu, Qingyao,Wu, Dianxing.

[9]Nitrogen losses from fertilizers applied to maize, wheat and rice in the North China Plain. Cai, GX,Chen, DL,Ding, H,Pacholski, A,Fan, XH,Zhu, ZL. 2002

[10]Fate of N-15 derived from composts and urea in soils under different long-term N management in pot experiments. Luo, LG,Kondo, M,Itoh, S.

[11]Biomass digestibility is predominantly affected by three factors of wall polymer features distinctive in wheat accessions and rice mutants. Wu, Zhiliang,Zhang, Mingliang,Wang, Lingqiang,Tu, Yuanyuan,Zhang, Jing,Xie, Guosheng,Zou, Weihua,Li, Fengcheng,Guo, Kai,Li, Qing,Peng, Liangcai,Wu, Zhiliang,Zhang, Mingliang,Wang, Lingqiang,Tu, Yuanyuan,Zhang, Jing,Xie, Guosheng,Zou, Weihua,Li, Fengcheng,Guo, Kai,Li, Qing,Peng, Liangcai,Wu, Zhiliang,Zhang, Mingliang,Wang, Lingqiang,Tu, Yuanyuan,Zhang, Jing,Xie, Guosheng,Zou, Weihua,Li, Fengcheng,Peng, Liangcai,Guo, Kai,Li, Qing,Gao, Chunbao. 2013

[12]How widespread are yield declines in long-term rice experiments in Asia?. Dobermann, A,Moya, P,Abdulrachman, S,Singh, B,Lal, P,Li, SY,Lin, B,Panaullah, G,Sariam, O,Singh, Y,Swarup, A,Tan, PS,Zhen, QX. 2000

[13]Do organic amendments improve yield trends and profitability in intensive rice systems?. Dobermann, A,Ladha, JK,Yadav, RL,Bao, L,Gupta, RK,Lal, P,Panaullah, G,Sariam, O,Singh, Y,Swarup, A,Zhen, QX. 2003

[14]The wheat durable, multipathogen resistance gene Lr34 confers partial blast resistance in rice. Krattinger, Simon G.,Sucher, Justine,Selter, Liselotte L.,Chauhan, Harsh,Keller, Beat,Zhou, Bo,Tang, Mingzhi,Upadhyaya, Narayana M.,Lagudah, Evans S.,Mieulet, Delphine,Guiderdoni, Emmanuel,Weidenbach, Denise,Schaffrath, Ulrich,Chauhan, Harsh.

[15]Inter-relationship between electrophoretic characteristics of pseudocereal and cereal proteins and their microscopic structure for possible substitution based on nutritional evaluation. Gorinstein, S,Yamamoto, K,Kobayashi, S,Taniguchi, H,Pawelzik, E,Delgado-Licon, E,Shaoxian, Y,Hongliang, S,Leticia, A,Ayala, M,Trakhtenberg, S.

[16]Rice black-streaked dwarf virus P10 induces membranous structures at the ER and elicits the unfolded protein response in Nicotiana benthamiana. Sun, Zongtao,Xie, Li,Sun, Liying,Li, Junmin,Wang, Xu,Chen, Jianping,Yang, Di,Zhang, Shanglin,Zhu, Qisong. 2013

[17]Nonstructural protein P7-2 encoded by Rice black-streaked dwarf virus interacts with SKP1, a core subunit of SCF ubiquitin ligase. Wang, Qian,Wang, Qian,Tao, Tao,Han, Yanhong,Chen, Xiangru,Fan, Zaifeng,Li, Dawei,Yu, Jialin,Han, Chenggui,Wang, Qian,Tao, Tao,Han, Yanhong,Chen, Xiangru,Fan, Zaifeng,Li, Dawei,Yu, Jialin,Han, Chenggui. 2013

[18]Dual transcriptome analysis reveals insights into the response to Rice black-streaked dwarf virus in maize. Zhou, Yu,Duan, Canxing,Hao, Zhuanfang,Li, Mingshun,Yong, Hongjun,Zhang, Degui,Zhang, Shihuang,Weng, Jianfeng,Li, Xinhai,Zhou, Yu,Xu, Zhennan,Wang, Zhenhua,Chen, Yanping,Meng, Qingchang,Wu, Jirong.

[19]Structure and components of the globular and filamentous viroplasms induced by Rice black-streaked dwarf virus. Xie, Li,Lv, Ming-fang,Song, Xi-Jiao,Yang, Jian,Li, Jing,Sun, Zong-Tao,Chen, Jian-Ping,Zhang, Heng-Mu,Lv, Ming-fang,Chen, Jian-Ping,Zhang, Ying-Yi. 2017

[20]Development and use of three monoclonal antibodies for the detection of rice black-streaked dwarf virus in field plants and planthopper vectors. Wu, Jianxiang,Ni, Yuequn,Liu, Huan,Rao, Lixia,Zhou, Xueping,Zhou, Yijun. 2013

作者其他论文 更多>>

微信小程序