DE NOVO TRANSCRIPTOME ANALYSIS OF MULBERRY (MORUS L.) UNDER DROUGHT STRESS USING RNA-SEQ TECHNOLOGY

文献类型： 外文期刊

第一作者： Wang, Heng

作者： Wang, Heng;Tong, Wei;Feng, Li;Jiao, Qian;Long, Li;Fang, Rongjun;Zhao, Weiguo;Long, Li;Zhao, Weiguo;Fang, Rongjun;Zhao, Weiguo

作者机构：

关键词： mulberry;drought stress;RNA-seq;de novo assembly;transcriptome;gene expression

期刊名称：RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY （ 影响因子：0.796； 五年影响因子：0.862 ）

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年卷期：

页码：

收录情况： SCI

摘要： A large-scale RNA sequencing (RNA-seq) of mulberry {Moms L.) was carried out between two samples in regular and drought stress condition. In this research, de novo assembly was performed, and totally 54736 contigs were obtained from the reads, including the scaffolded regions. 1051 genes were identified that were significantly differently expressed between the two samples. As determined by Gene Ontology (GO) annotation and the Kyoto Encyclopedia of Genes and Genomes pathway mapping, 10110 GO terms and 247 pathways were assigned and then analyzed. Thousands of SSR markers produced in this study will enable genetic linkage mapping construction and gene-based association studies. Seven unique genes showing different expression level in control and drought stress groups were subsequently analyzed and identified by real-time PCR. For lack of mulberry whole genome information, transcriptome and de novo analysis from the two samples will provide important and useful information for later research and helpgenetic breeding of mulberry.

分类号： Q50

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