Polymorphic microsatellite markers in Thrips hawaiiensis (Thysanoptera: Thripidae)

文献类型： 外文期刊

第一作者： Wu, Yi

作者： Wu, Yi;Liu, Kui;Qiu, Haiyan;Wu, Yi;Li, Fujun;Cao, Yang

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期刊名称：APPLIED ENTOMOLOGY AND ZOOLOGY （ 影响因子：1.403； 五年影响因子：1.381 ）

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收录情况： SCI

摘要： Thrips hawaiiensis (Morgan, 1913) is an important insect pest of fruits and vegetables. At present, it is primarily distributed in tropical and subtropical area. For a better understanding of the genetic makeup and migration patterns of T. hawaiiensis throughout the world, we isolated 11 novel polymorphic microsatellite loci from an enriched genomic library based on a biotin/streptavidin capture protocol. Genetic parameters were estimated on 80 individual thrips from two natural populations. The results showed that all 11 loci were highly polymorphic; the number of alleles ranged from six to 37, and nine loci demonstrated polymorphic information content (PIC) > 0.5. The observed (H-O) and expected (H-E) heterozygosities ranged from 0.350 to 0.925 and 0.307 to 0.952, respectively. Furthermore, only four locus/population combinations significantly deviated from Hardy-Weinberg equilibrium (HWE). These microsatellite markers have potential utility in population structure and gene flow studies of this species

分类号： Q96

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