Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle
文献类型: 外文期刊
第一作者: Li, Z.
作者: Li, Z.;Huang, H.;Zhao, H.;Meng, K.;Shi, P.;Yang, P.;Luo, H.;Wang, Y.;Yao, B.;Li, Z.;Zhao, J.
作者机构:
关键词: clone library;cysteine phytase;rumen;Small Tail Han sheep;transcriptional profile
期刊名称:LETTERS IN APPLIED MICROBIOLOGY ( 影响因子:2.858; 五年影响因子:2.776 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Cysteine phytase is the main phytate-degrading enzyme of ruminant animals. To explore the genetic diversity and dynamic expression profile of cysteine phytase in sheep rumen during a feeding cycle, four transcript (0, 4, 9 and 16h after feeding) and one DNA (9h after feeding) clone libraries were constructed, respectively. A total of 46 distinct gene fragments were identified, and most of these sequences had low identities (<60%) with known phytases. Great divergence was found in the constitution and abundance of genes at the genome and transcriptional levels, and the transcript data are more reliable to reflect the information of functional genes. Phylogenetic analysis indicated that the genes from uncultured bacteria instead of Firmicutes played the major phytate-degrading role. Further comparative analysis revealed the dynamic constitution of cysteine phytase genes in rumen at different time points.
分类号: Q939
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