Allelic-specific expression in relation to Bombyx mori resistance to Bt toxin

文献类型： 外文期刊

第一作者： Chen, Yazhou

作者： Chen, Yazhou;Islam, Iftakher;You, Lang;Wang, Yueqiang;Li, Zhiqian;Ling, Lin;Zeng, Baosheng;Xu, Jun;Huang, Yongping;Tan, Anjiang;Li, Muwang;Li, Muwang

作者机构：

关键词： Bt toxin;Allelic-specific expression trans-regulatory;SNPs;Insect resistance;Bombyx mori

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Understanding the mechanism of Bt resistance is one of the key elements of the effective application of Bt in pest control. The lepidopteran model insect, the silkworm, demonstrates qualities that make it an ideal species to use in achieving this understanding. We screened 45 strains of silkworm (Bombyx man) using a Cry1Ab toxin variant. The sensitivity levels of the strains varied over a wide range. A resistant strain (P50) and a phylogenetically related susceptible strain (Dazao) were selected to profile the expressions of 12 Bt resistance-related genes. The SNPs in these genes were detected based on EST analysis and were validated by allelic-specific PCR. A comparison of allelic-specific expression between P50 and Dazao showed that the transcript levels of heterozygous genes containing two alleles rather than an imbalanced allelic expression contribute more to the resistance of P50 against Bt. The responses of the allelic-specific expression to Bt in hybrid larvae were then investigated. The results showed that the gene expression pattern of an ATP-binding cassette transporter C2 (ABCC2) and an aminopeptidase N (APN3), changed in an allelic-specific manner, with the increase of the resistant allele expression correlated with larval survival. The results suggest that a trans-regulatory mechanism in ABCC2 and APN3 allelic-specific expression is involved in the insect's response to the Bt toxin. The potential role of allelic-specific gene regulation in insect resistance to Bt toxins is discussed. (C) 2014 Elsevier Ltd. All rights reserved.

分类号： Q5

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