Allelic-specific expression in relation to Bombyx mori resistance to Bt toxin

文献类型： 外文期刊

第一作者： Chen, Yazhou

作者： Chen, Yazhou;Islam, Iftakher;You, Lang;Wang, Yueqiang;Li, Zhiqian;Ling, Lin;Zeng, Baosheng;Xu, Jun;Huang, Yongping;Tan, Anjiang;Li, Muwang;Li, Muwang

作者机构：

关键词： Bt toxin;Allelic-specific expression trans-regulatory;SNPs;Insect resistance;Bombyx mori

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Understanding the mechanism of Bt resistance is one of the key elements of the effective application of Bt in pest control. The lepidopteran model insect, the silkworm, demonstrates qualities that make it an ideal species to use in achieving this understanding. We screened 45 strains of silkworm (Bombyx man) using a Cry1Ab toxin variant. The sensitivity levels of the strains varied over a wide range. A resistant strain (P50) and a phylogenetically related susceptible strain (Dazao) were selected to profile the expressions of 12 Bt resistance-related genes. The SNPs in these genes were detected based on EST analysis and were validated by allelic-specific PCR. A comparison of allelic-specific expression between P50 and Dazao showed that the transcript levels of heterozygous genes containing two alleles rather than an imbalanced allelic expression contribute more to the resistance of P50 against Bt. The responses of the allelic-specific expression to Bt in hybrid larvae were then investigated. The results showed that the gene expression pattern of an ATP-binding cassette transporter C2 (ABCC2) and an aminopeptidase N (APN3), changed in an allelic-specific manner, with the increase of the resistant allele expression correlated with larval survival. The results suggest that a trans-regulatory mechanism in ABCC2 and APN3 allelic-specific expression is involved in the insect's response to the Bt toxin. The potential role of allelic-specific gene regulation in insect resistance to Bt toxins is discussed. (C) 2014 Elsevier Ltd. All rights reserved.

分类号： Q5

  • 相关文献

[1]Bacillus thuringiensis insecticidal crystal proteins affect lifespan and reproductive performance of helicoverpa armigera and spodoptera exigua adults. YING ZHANG,YAN MA,PIN-JUN WAN,LI-LI MU,GUO-QING LI.

[2]Effects of the consecutive cultivation and periodic residue incorporation of Bacillus thuringiensis (Bt) cotton on soil microbe-mediated enzymatic properties. Zhenhua Chen,Chen, Lijun,Kai Wei,Lijun Chen,Zhijie Wu,Junyu Luo,Jinjie Cui.

[3]Marker-free, tissue-specific expression of Cry1Ab as a safe transgenic strategy for insect resistance in rice plants. Qi, Yongbin,Chen, Lei,Jin, Qingsheng,Zhang, Xiaoming,Qi, Yongbin,He, Zuhua,He, Xiuling,He, Zuhua. 2013

[4]Impacts of transgenic poplar-cotton agro-ecosystems upon target pests and non-target insects under field conditions. Zhang, D. J.,Liu, J. X.,Li, C. L.,Yang, M. S.,Zhang, D. J.,Lu, Z. Y.,Comada, E.. 2015

[5]Frequency of Bt resistance alleles in Helicoverpa armigera in 2007-2009 in the Henan cotton growing region of China. Feng, Hongqiang,Qiu, Feng,Gao, Yulin.

[6]Expression of cadherin, aminopeptidase N and alkaline phosphatase genes in Cry1Ac-susceptible and Cry1Ac-resistant strains of Plutella xylostella (L.). Yang, Z-X.,Wu, Q-J.,Wang, S-L.,Chang, X-L.,Wang, J-H.,Guo, Z-J.,Lei, Y-Y.,Xu, B-Y.,Zhang, Y-J.,Yang, Z-X.. 2012

[7]Changes in susceptibility to conventional insecticides of a Cry1Ac-selected population of Helicoverpa armigera (Hubner) (Lepidoptera : Noctuidae). Guo, YY. 2004

[8]Genetic analysis and pyramiding of two gall midge resistance genes (Gm-2 and Gm-6t) in rice (Oryza sativa L.). Katiyar, S,Verulkar, S,Chandel, G,Zhang, Y,Huang, B,Bennett, J. 2001

[9]Aspects of soybean insect resistance breeding in China. Wang, S. 2004

[10]RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper (Nilaparvata lugens). Xu, XF,Mei, HW,Luo, LJ,Cheng, XN,Li, ZK. 2002

[11]Quantitative Trait Loci for Asian Corn Borer Resistance in Maize Population Mc37 x Zi330. Li Xia,He Kang-lai,Wang Zhen-ying,Bai Shu-xiong,Li Xia. 2010

[12]Development and bioassay of transgenic Chinese cabbage expressing potato proteinase inhibitor II gene. Zhang, Junjie,Liu, Fan,Yao, Lei,Yin, Yue,Wang, Guixiang,Zhang, Junjie,Huang, Yubi,Luo, Chen. 2012

[13]Studies on insect resistance of Bt transplastomic plants and the phenotype of their progenies. Zhang, ZL,Chen, X,Qian, KX,Shen, GF. 1999

[14]A novel synthetic Cry1Ab gene resists rice insect pests. Song, F. S.,Song, F. S.,Ni, D. H.,Li, H.,Duan, Y. B.,Yang, Y. C.,Ni, J. L.,Lu, X. Z.,Wei, P. C.,Li, L.,Yang, J. B.,Li, H.,Wei, P. C.. 2014

[15]Expressing a modified cowpea trypsin inhibitor gene to increase insect tolerance against Pieris rapae in Chinese cabbage. Ma, Xiaoli,Pei, Yanxi,Ma, Xiaoli,Zhu, Zhen,Li, Yane,Yang, Guangdong. 2017

[16]Development of insect-resistant transgenic cotton with chimeric TVip3A*accumulating in chloroplasts. Wu, Jiahe,Zhang, Xiangrong,Tian, Yingchuan,Luo, Xiaoli,Shi, Yuejing. 2011

[17]Effects of different brush border membrane vesicle isolation protocols on proteomic analysis of Cry1Ac binding proteins from the midgut of Helicoverpa armigera. Liang, Ge-Mei,Zhang, Jie,Wu, Kong-Ming,Guo, Yu-Yuan,Rector, Brian G..

[18]Vacuum infiltration transformation of non-heading Chinese cabbage (Brassica rapa L. ssp chinensis) with the pinII gene and bioassay for diamondback moth resistance. Zhang, Junjie,Liu, Fan,Zhang, Junjie,Huang, Yubi,Yao, Lei,Zhao, Qing,Luo, Chen.

[19]Cross-resistance studies of Cry1Ac-resistant strains of Helicoverpa armigera (Lepidoptera : Noctuidae) to Cry2Ab. Luo, Shudong,Wu, Kongming,Tian, Yan,Liang, Gemei,Feng, Xue,Zhang, Jie,Guo, Yuyuan.

[20]Enhanced yield performance of Bt rice under target-insect attacks: implications for field insect management. Xia, Hui,Lu, Bao-Rong,Xu, Kai,Wang, Wei,Yang, Xiao,Yang, Chao,Ye, Wenli,Luo, Ju,Lai, Fengxiang,Fu, Qiang.

作者其他论文 更多>>

微信小程序