An AFLP-based approach for the identification of sex-linked markers in Amur sturgeon Acipenser schrenckii Brandt, 1869
文献类型： 外文期刊
第一作者： Xiao, T. -Q.
作者： Xiao, T. -Q.;Lu, C. -Y.;Li, C.;Cheng, L.;Cao, D. -C.;Sun, X. -W.;Xiao, T. -Q.
作者机构：
期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Sturgeon species are widely known for their caviars. A large part of the expense associated with caviar production is due to the length of time individuals must be reared before reaching maturity. Sex identification at an early age would reduce costs of broodstock rearing. A DNA AFLP-fingerprinting approach was used to identify sex-specific markers and sex-associated markers that exhibit a difference between female and male Amur sturgeon Acipenser schrenckii Brandt, 1869. AFLP analysis of six females and six males using 64 primer combinations yielded a total of 7116 AFLP bands of which 1264 (17.76%) bands were polymorphic. The polymorphism exhibited differences between female (15.64%) and male (16.34%) groups. Sex-specific markers were not detected among the AFLP bands. However, 42 presumed sex-associated bands that exhibited large differences between females and males were identified in 34 primer combinations. None of the 28 SCAR markers derived from the 39 sequences exhibited sex specificity when tested against 15 females and 15 males. Nevertheless, five SCAR markers exhibited a significant difference between the sexes. Sequence analysis using blastn in GenBank revealed high homology for 10 fragments, among which C4 was associated with the secretion of prolactin in mammals, but was not sex-specific based on testing of SCAR markers. The five SCAR markers and the clone C4 may represent candidate genes for future research of sex specificity.
分类号： S9
- 相关文献
作者其他论文 更多>>
-
Characterization of a Novel C-type Lectin against OsHV-1 infection in Scapharca broughtonii
作者：Wang, D.;Zhou, H.;Wang, D.;Huang, B.;Yu, J.;Bai, C.;Li, C.;Wang, C.;Xin, L.;Wang, D.;Huang, B.;Yu, J.;Bai, C.;Li, C.;Wang, C.;Xin, L.;Yu, J.
关键词：Scapharca broughtonii; C-type lectin; PAMP binding; OsHV-1 infection
-
Identification and Analysis of Proteins and RNAs Interacting with a PPR Protein OsNPPR1 in Rice
作者：Hao, Y.;Zhao, X.;Huang, F.;Li, C.;Zhu, Y.
关键词：Oryza sativa; OsNPPR1; nuclear PPR proteins; transcription and post-transcription regulation; mitochondria function
-
Genome-scale and multi-gene phylogenetic analyses of Colletotrichum spp. host preference and associated with medicinal plants
作者：Zhang, Q.;Nizamani, M. M.;Yang, Y. Q.;Wang, Y.;Li, C.;Feng, Y.;Jayawardena, R. S.;Hyde, K. D.;Jayawardena, R. S.;Hyde, K. D.;Feng, Y.
关键词：Comparative genomics; Genome prediction; Multi-locus phylogeny; Phylogenomics; Seven new species
-
OPTIMAL PLANTING DENSITY FOR ACHIEVEING HIGHER YIELD AND FIBER QUALITY OF TWO TYPES OF COTTON (GOSSYPIUM HIRSUTUM L.)
作者：Ye, S.;Liu, F.;Tian, C.;Li, C.;Han, W.;Jiang, C.;Wu, D.
关键词：cotton variety; fruit branch; fiber quality; lint yield; micronaire value
-
Screening of highly efficient photosynthetic hybrids of Oryza officinalis and analysis of their photosynthetic pathway genes
作者：Li, E. X.;Yin, F. Y.;Xiao, S. Q.;Cheng, Z. Q.;Ke, X.;Kan, D. Y.;Li, C.;You, J. H.
关键词：excellent traits; gene homology; PCR amplification; photosynthetic parameters; primer design
-
Biological control of Qinghai plateau terrestrial weeds with the A. alternata HL-1
作者：Cheng, L.;Zhu, H. X.;Wei, Y. H.;Guo, L. Z.;Weng, H.;Guo, Q. Y.;Cheng, L.;Zhu, H. X.;Wei, Y. H.;Guo, L. Z.;Weng, H.;Guo, Q. Y.;Cheng, L.;Zhu, H. X.;Wei, Y. H.;Guo, L. Z.;Weng, H.;Guo, Q. Y.
关键词：Endophytic fungi; Weeds; Herbicidal activity; Crop safety; Fermentation; Optimization; Formulation
-
THE ABILITY OF LACTOBACILLUS RHAMNOSUS TO BIND PATULIN AND ITS APPLICATION IN APPLE JUICE
作者：Li, J.;Liu, L.;Liu, L.;Li, C.;Tan, Y.;Meng, Y.
关键词：Lactobacillus rhamnosus; patulin; adsorption; complex; apple juice
