Immunoglobulin G binding protein (IGBP) from Rhipicephalus haemaphysaloides: identification, expression, and binding specificity

文献类型： 外文期刊

第一作者： Gong, Haiyan

作者： Gong, Haiyan;Qin, Shunqing;Wan, Xiuhong;Zhang, Houshuang;Zhou, Yongzhi;Cao, Jie;Zhou, Jinlin;Xuan, Xuenan;Suzuki, Hiroshi;Zhou, Jinlin

作者机构：

关键词： Immunoglobulin G binding protein (IGBP) Rhipicephalus haemaphysaloides;Binding specificity

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.289； 五年影响因子：2.403 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： As the second most important human ectoparasite, ranked only after mosquitoes, the tick threatens the development of husbandry and even the health of humans worldwide. Immunoglobulin G binding proteins (IGBPs) are considered to be the major factors used by ticks to evade the host immune system and the damage caused by host antibodies. In this study, an IGBP-MB homologue was identified in the tick Rhipicephalus haemaphysaloides, which was predominantly detected in the salivary glands and hemolymph of male ticks. Recombinant IGBP (rIGBP/His) displayed significant binding activity to IgGs from rabbits and pigs, and bound to the F(ab)'2 but not the Fc fragment of rabbit IgG. Although the silencing of IGBP expression in ticks had no obvious effect on their blood-feeding and subsequent oviposition, antibodies raised to rIGBP/GST reduced the replete body weight (218.9±20 mg in the control group vs. 142.5±43.3 mg in the test group, P<0.05 by Student's t test) and increased the mortality of the ticks. This study extends our understanding of the immunoevasive function of IGBPs and is a step towards the development of a vaccine against ticks.

分类号： R53

  • 相关文献

[1]Binding Characterization of Recombinant Odorant-binding Proteins from the Parasitic Wasp, Microplitis mediator (Hymenoptera: Braconidae). Zhang, Shuai.

[2]Functional characterizations of one odorant binding protein and three chemosensory proteins from Apolygus lucorum (Meyer-Dur) (Hemiptera: Miridae) legs. Jin-Feng Hua,Shuai Zhang,Jin-Jie Cui,Dao-Jie Wang,Chun-Yi Wang,Jun-Yu Luo,Li-Min Lv,Yan Ma. 2013

作者其他论文 更多>>

微信小程序