Cloning, expression and methylation analysis of piwil2 in half-smooth tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Zhang, Liyan

作者： Zhang, Liyan;Zhao, Wen;Zhang, Liyan;Liu, Wanjun;Shao, Changwei;Zhang, Ning;Li, Hailong;Liu, Kun;Dong, Zhongdian;Qi, Qian;Chen, Songlin;Liu, Wanjun;Dong, Zhongdian;Qi, Qian

作者机构：

关键词： Histological observations;Phylogenetic analysis;RACE;RT-qPCR;Spermatogenesis

期刊名称：MARINE GENOMICS （ 影响因子：1.71； 五年影响因子：1.969 ）

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收录情况： SCI

摘要： piwi is an important regulator gene in germ cell division during spermatogenesis. piwi homologous genes are involved in gametogenesis and germline specification, and knocking down these genes could affect germ cell meiotic progression. To understand the function of piwi-related genes in spermatogenesis, we cloned a Piwi-subfamily member (piwil2 gene) from the gonad of Cynoglossus semilaevis. The full-length of piwil2 cDNA was 3314 bp, including a 3162 bp open reading frame (ORF), a 60 bp 5'-UTR, along with a 92 bp 3'-UTR, and encoded a predicted protein of 1053 amino acid residues. Phylogenetic analysis showed that the PIWIL2 putative protein belonged to the Argonaute protein family, and Piwi-subfamily, with typical PAZ and Piwi domains. Ultrathin sections of different gonadal stages, and real-time quantitative PCR showed that the relative expression of the piwil2 gene couldn't be detected until day 95 (95 days) larvae, when germ cell divided rapidly in C semilaevis. The piwil2 transcripts were more abundant in gonads of males and neo-males than in females, and weakly expressed in other tissues and organs. Compared with the relative expression of piwil2 in gonads, the CpG methylation levels were significantly higher in females. Chromosomal fluorescence in situ hybridization (FISH) showed that the piwil2 gene was located on the Z sex chromosome of C semilaevis. These results suggest that piwil2 plays an important role in spermatogenesis of C semilaevis. (c) 2014 Elsevier B.V. All rights reserved.

分类号： Q3

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