Entry of Bombyx mori nucleopolyhedrovirus into BmN cells by cholesterol-dependent macropinocytic endocytosis

文献类型: 外文期刊

第一作者: Huang, Jinshan

作者: Huang, Jinshan;Hao, Bifang;Cheng, Chen;Liang, Fei;Shen, Xingjia;Huang, Jinshan;Hao, Bifang;Shen, Xingjia;Cheng, Xiaowen

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关键词: BmNPV;Macropinocytosis;Endocytosis;Cholesterol

期刊名称:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( 影响因子:3.575; 五年影响因子:3.381 )

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收录情况: SCI

摘要: Bombyx mori nucleopolyhedrovirus (BmNPV) is a serious viral pathogen of silkworm, and no drug or specific protection against BmNPV infection is available at present time. Although functions of most BmNPV genes were depicted in recent years, knowledge on the mechanism of BmNPV entry into insect cells is still limited. Here BmNPV cell entry mechanism is investigated by different endocytic inhibitor application and subcellular analysis. Results indicated that BmNPV enters BmN cells by clathrin-independent macropinocytic endocytosis, which is mediated by cholesterol in a dose-dependent manner, and cholesterol replenishment rescued the BmNPV infection partially. (C) 2014 Elsevier Inc. All rights reserved.

分类号: Q5

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