Nup358 and Nup153 Facilitate nuclear import of BmNPV nucleocapsids in Bombyx mori cells

文献类型： 外文期刊

第一作者： Yin, Kangping

作者： Yin, Kangping;Zhang, Tianran;Huang, Jinshan;Hao, Bifang;Huang, Jinshan;Hao, Bifang

作者机构：

关键词： BmNPV; Baculovirus; NPC; Nuclear import; Nucleoporins

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.4； 五年影响因子：3.0 ）

ISSN： 0022-2011

年卷期： 2025 年 211 卷

页码：

收录情况： SCI

摘要： Nuclear pore complexes (NPCs) are crucial for the nuclear import of viral genomes, serving as gateways for many viruses to deliver their genetic material into host cell nuclei. However, the role of NPCs in the entry of Bombyx mori nucleopolyhedrovirus (BmNPV) remains poorly understood. This study investigates the involvement of specific nucleoporins in the nuclear import of BmNPV nucleocapsids, a crucial step for viral replication in host cells. Using a combination of Importazole, wheat germ agglutinin (WGA), and small interfering RNAs (siRNAs), we demonstrate that BmNPV entry into BmN cells is mediated through the NPC. Importazole and WGA treatment significantly inhibited viral infection, highlighting the essential role of nucleoporins in BmNPV entry. Additionally, siRNA-mediated knockdown of Nup358 and Nup153 resulted in a marked accumulation of nucleocapsids in the cytoplasm. Overexpression of the N-terminal fragment of Nup358 (1-1127) enhanced nucleocapsid entry efficiency, whereas deletion of the phenylalanine-glycine (FG) repeats in Nup153 (Nup153 Delta FG) reduced viral entry. These results confirm the pivotal roles of Nup358 and Nup153 in the nuclear import of BmNPV nucleocapsids. Our findings provide new insights into the molecular interactions between BmNPV and host NPCs, offering potential targets for controlling BmNPV infections in sericulture.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序