Inducible and constitutive expression of an elicitor gene Hrip1 from Alternaria tenuissima enhances stress tolerance in Arabidopsis
文献类型: 外文期刊
第一作者: Qiu, De-Wen
作者: Qiu, De-Wen;Zeng, Hong-Mei;Guo, Li-Hua;Yang, Xiu-Fen;Liu, Zheng
作者机构:
关键词: Protein elicitor;Hrip1;Transgenic Arabidopsis;Drought stress;Salt stress;Botrytis
期刊名称:TRANSGENIC RESEARCH ( 影响因子:2.788; 五年影响因子:2.377 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Hrip1 is a novel hypersensitive response-inducing protein secreted by Alternaria tenuissima that activates defense responses and systemic acquired resistance in tobacco. This study investigates the role that Hrip1 plays in responses to abiotic and biotic stress using transgenic Arabidopsis thaliana expressing the Hrip1 gene under the control of the stress-inducible rd29A promoter or constitutive cauliflower mosaic virus 35S promoter. Bioassays showed that inducible Hrip1 expression in rd29Aa center dot Hrip1 transgenic lines had a significantly higher effect on plant height, silique length, plant dry weight, seed germination and root length under salt and drought stress compared to expression in 35Sa center dot Hrip1 lines and wild type plants. The level of enhancement of resistance to Botrytis cinerea by the 35Sa center dot Hrip1 lines was higher than in the rd29Aa center dot Hrip1 lines. Moreover, stress-related gene expression in the transgenic Arabidopsis lines was significantly increased by 200 mM NaCl and 200 mM mannitol treatments, and defense genes in the jasmonic acid and ethylene signaling pathway were significantly up-regulated after Botrytis inoculation in the Hrip1 transgenic plants. Furthermore, the activity of some antioxidant enzymes, such as peroxidase and catalase increased after salt and drought stress and Botrytis infection. These results suggested that the Hrip1 protein contributes to abiotic and biotic resistance in transgenic Arabidopsis and may be used as a useful gene for resistance breeding in crops. Although the constitutive expression of Hrip1 is suitable for biotic resistance, inducible Hrip1 expression is more responsive for abiotic resistance.
分类号: R394
- 相关文献
作者其他论文 更多>>
-
Species-specific IL-1β is an inflammatory sensor of Seneca Valley Virus 3C Protease
作者:Huang, Xiangyu;Zhao, Zhenchao;Chai, Lvye;Yan, Ya;Yuan, Ye;Wu, Lei;Li, Minjie;Jiang, Xiaohan;Li, Xin;Huang, Xiangyu;Zhao, Zhenchao;Chai, Lvye;Yan, Ya;Yuan, Ye;Wu, Lei;Li, Minjie;Jiang, Xiaohan;Li, Xin;Zhu, Cheng;Wang, Haiwei;Liu, Zheng;Li, Pingwei
关键词:
-
Identification and bioactivity evaluation of ring-opening and lactone forms of enterolactone from sheep fed flaxseed cake
作者:Zhuang, Cong-Cong;Liu, Chun-Rui;Xu, Hai-Yan;Yang, Xin-Yue;Wu, Dan-Dan;Zheng, Zhong;Wang, Li-Wei;Ma, Chao-Mei;Wang, Li-Wei;Liu, Zheng
关键词:Mammalian lignan; ring-opening enterolactone; pH dependent structure; breast cancer; anti-osteoporosis
-
Identification and genetic diversity analysis of broomrape in Xinjiang, China
作者:Zhang, Xuekun;Du, Juan;Wang, Panpan;Liu, Zheng;Yao, Zhaoqun;Zhao, Sifeng;Xi, Hui;Wang, Peng
关键词:Broomrape; Phylogenetic analysis; Genetic diversity; ISSR
-
Suitability Analysis of Kitchen Waste Compost for Rice Seedling Substrate Preparation
作者:Ge, Liqun;Liu, Zheng;Liu, Xinyu;Zhou, Yaohua;Jia, Ke;Yan, Liping;Dong, Xue;Pan, Rongguang;Liu, Xinyu;Li, Shuang
关键词:kitchen waste compost; rice; seedling substrate; cost
-
Study on the Hydrodynamic Performance of the Beam Used in the Antarctic Krill Beam Trawl
作者:Li, Yuyan;Wang, Zhongqiu;Zhang, Xun;Wang, Lumin;Zhang, Yu;Ma, Shuo;Qi, Guangrui;Wang, Yongjin;Liu, Zheng
关键词:trawl beam; Antarctic krill trawl; hydrodynamic performance; flume test; numerical simulation
-
Comparative physiological, metabolomic and transcriptomic analyses reveal the mechanisms of differences in pear fruit quality between distinct training systems
作者:Liu, Zheng;Li, Xie-Yu;Yang, Li;Cheng, Yin-Sheng;Nie, Xian-Shuang;Wu, Tao
关键词:Pear fruits; Training systems; Canopy environment; Metabolome; Transcriptome; Phenylpropanoid biosynthesis pathway
-
Aptamer functionalized magnetic hydrophobic polymer with synergetic effect for enhanced adsorption of alternariol from wheat
作者:Zhao, Liping;Wang, Xinjie;Yang, Ruiqi;Luan, Yunxia;Li, Linsen;Liu, Zheng;Luo, Zhaofeng;Fang, Xiaona
关键词:Alternariol adsorption; Capture-SELEX; Aptamer; Magnetic hydrophobic polymer; Synergistic effect