Preparation and characterization of ultrasensitive and specific polyclonal antiserum against ciprofloxacin based on cationized bovine serum albumin

文献类型： 外文期刊

第一作者： Deng, Sheng-Liang

作者： Deng, Sheng-Liang;Li, Ping;Liu, Hong-Bin;Yang, Shu-Ming;Deng, Sheng-Liang;Li, Ping

作者机构：

关键词： ciprofloxacin;cationized BSA;polyclonal antiserum;heterologous ELISA

期刊名称：CHEMICAL PAPERS （ 影响因子：2.097； 五年影响因子：1.831 ）

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收录情况： SCI

摘要： An ultrasensitive polyclonal antiserum against ciprofloxacin (CPFX) was developed by introducing cationized bovine serum albumin (BSA) as the carrier. Conjugates used as immunogens were synthesized using CPFX coupled to BSA cationized with ethylenediamine and hexamethylenediamine, namely CPFX-eBSA and CPFX-hBSA. The results showed that the antisera immunized with CPFX-hBSA exhibit an improved sensitivity and specificity compared with those immunized with CPFX-eBSA. Under the conditions of optimal heterologous enzyme-linked immunosorbent assay, the optimal antiserum yielded an IC50 of 0.097 ng mL(-1), which proves it to be more sensitive than any CPFX antibody. Cross-reactivities with norfloxacin, enrofloxacin, and ofloxacin were < 3.0 %. No cross-reactivities with penicillin and gentamicin were found. The limit of detection was 0.01 ng mL(-1), which is far below the maximum residue level established by the EU regulation, suggesting the great potential of the presented antiserum for quantitative assays of CPFX in samples.

分类号： O6

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