Analysis of (all-E)-lutein and its (Z)-isomers during illumination in a model system

文献类型： 外文期刊

第一作者： Li, Dajing

作者： Li, Dajing;Xiao, Yadong;Zhang, Zhongyuan;Liu, Chunquan;Xiao, Yadong;Li, Dajing

作者机构：

关键词： (All-E)-lutein;Light;Model;Isomerization;Degradation

期刊名称：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS （ 影响因子：3.935； 五年影响因子：3.563 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Light induced-isomerization of (all-E)-lutein in organic solvent and starch model systems was investigated. Lutein and its (Z)-isomers were separated by HPLC using a C-30 column and gradient mobile phase based on methanol-methyl-tert-butyl ether-water in 24 min. (All-E)-lutein and twelve (Z)-isomers of lutein, in addition a small amount of (all-E)-zeaxanthin and (9Z, 9'Z)-zeaxanthin were identified by HPLC-DAD-APCI-MS. Five di-(Z)-luteins were identified for the first time, namely, (9Z, 9'Z)-, (9Z, 13Z)/(9Z, 13'Z)-, (13Z, 15Z)- and (9Z, 15Z)-lutein and (9Z, 9'Z)-zeaxanthin. A mixture of (9'Z)-lutein and of (9'Z)lutein was the main product of the iodine-catalyzed photo-isomerization. (9Z, 13Z)/(9Z, 13'Z)-lutein were the major di-(Z)-isomers of lutein formed. The susceptibility of lutein to degradation was much less under dark storage than under lighted storage in starch model system. Isomerization and degradation of lutein and its (Z)-isomers proceeded simultaneously in all the model systems. (C) 2014 Elsevier B.V. All rights reserved.

分类号： R9

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