Specific ligands for classical swine fever virus screened from landscape phage display library

文献类型： 外文期刊

第一作者： Yin, Long

作者： Yin, Long;Luo, Yuzi;Du, Min;Qiu, Hua-Ji;Yin, Long;Liang, Bo;Wang, Fei;Liu, Aihua;Yin, Long;Wang, Fei;Liu, Aihua;Petrenko, Valery A.

作者机构：

关键词： Classical swine fever virus;E2 protein;Phage display;Specific iigand;Landscape phage display library;Antiviral activity

期刊名称：ANTIVIRAL RESEARCH （ 影响因子：5.97； 五年影响因子：5.801 ）

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收录情况： SCI

摘要： Classical swine fever (CSF) is a devastating infectious disease caused by classical swine fever virus (CSFV). The screening of CSFV-specific ligands is of great significance for diagnosis and treatment of CSF. Affinity selection from random peptide libraries is an efficient approach to discover ligands with high stability and specificity. Here, we screened phage ligands for the CSFV E2 protein from f8/8 landscape phage display library by biopanning and obtained four phage clones specific for the E2 protein of CSFV. Viral blocking assays indicated that the phage clone displaying the octapeptide sequence DRATSSNA remarkably inhibited the CSFV replication in PK-15 cells at a titer of 1010 transduction units, as evidenced by significantly decreased viral RNA copies and viral titers. The phage-displayed E2-binding peptides have the potential to be developed as antivirals for CSF.

分类号： R37

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