Isolation and characterization of an antifungal protein from Bacillus licheniformis HS10

文献类型: 外文期刊

第一作者: Wang, Zhixin

作者: Wang, Zhixin;Zheng, Li;Yang, Xiaona;Liu, Hongxia;Guo, Jianhua;Wang, Zhixin;Zheng, Li;Yang, Xiaona;Liu, Hongxia;Guo, Jianhua;Wang, Zhixin;Zheng, Li;Yang, Xiaona;Liu, Hongxia;Guo, Jianhua;Wang, Yunpeng;Zheng, Li;Zheng, Li

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关键词: Biocontrol;Bacillus licheniformis;Antifungal protein;Chromatography

期刊名称:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( 影响因子:3.575; 五年影响因子:3.381 )

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收录情况: SCI

摘要: Bacillus licheniformis HS10 is a good biocontrol agent against Pseudoperonospora cubensis which caused cucumber downy disease. To identify and characterize the antifungal proteins produced by B. licheniformis HS10, the proteins from HS10 were isolated by using 30-60% ammonium sulfate precipitation, and purified with column chromatography on DEAE Sepharose Fast Flow, RESOURCE Q and Sephadex G-75. And the SDS-PAGE and MALDI-TOF/TOF-MS analysis results demonstrated that the antifungal protein was a monomer with molecular weight of about 55 kDa, identified as carboxypeptidase. Our experiments also showed that the antifungal protein from B. licheniformis HS10 had significantly inhibition on eight different kinds of plant pathogenic fungi, and it was stable with good biological activity at as high as 100 degrees C for 30 min and in pH value ranged from 6 to 10. The biological activity was negatively affected by protease K and 10 mM metal cations except Ca2+. (C) 2014 Elsevier Inc. All rights reserved.

分类号: Q5

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