Construction of a standard reference plasmid containing seven target genes for the detection of transgenic cotton
文献类型: 外文期刊
第一作者: Wang, Xujing
作者: Wang, Xujing;Tang, Qiaoling;Dong, Lei;Dong, Yufeng;Su, Yueyan;Jia, Shirong;Wang, Zhixing
作者机构:
关键词: Standard reference plasmid;DNA construction;Transgenic cotton;Real-time PCR
期刊名称:PLASMID ( 影响因子:3.466; 五年影响因子:2.74 )
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收录情况: SCI
摘要: Insect resistance and herbicide tolerance are the dominant traits of commercialized transgenic cotton. In this study, we constructed a general standard reference plasmid for transgenic cotton detection. Target genes, including the cowpea trypsin gene cptI, the insect resistance gene cry1Ab/1Ac, the herbicide tolerance gene cp4-epsps, the Agrobacterium tumefaciens nopaline synthase (Nos) terminator that exists in transgenic cotton and part of the endogenous cotton SadI gene were amplified from plasmids pCPT1, pBT, pCP4 and pBI121 and from DNA of the nontransgenic cotton line K312, respectively.
分类号: R394
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