An atypical NLR pair TdCNL1/TdCNL5 from wild emmer confers powdery mildew resistance in wheat

文献类型： 外文期刊

第一作者： Zhu, Keyu

作者： Zhu, Keyu;Li, Miaomiao;Dong, Lingli;Zhang, Huaizhi;Lu, Ping;Xie, Jingzhong;Guo, Guanghao;Hou, Yikun;Yang, Yijun;Qiu, Dan;Wang, Gaojie;Huang, Baoge;Cui, Xuejia;Fu, Hongkui;Liu, Zhiyong;Zhang, Deyun;Wu, Qiuhong;Chen, Yongxing;Li, Beibei;Li, Wenling;Dong, Lei;Li, Hongjie;Hua, Wei;Zhang, Panpan;Hou, Yikun;Huang, Baoge;Cui, Xuejia;Fu, Hongkui;Liu, Zhiyong;Yuan, Chengguo;Fahima, Tzion;Nevo, Eviatar;Rong, Junkang;Hua, Wei;Liu, Zhiyong

作者机构：

期刊名称：NATURE GENETICS （ 影响因子：29.0； 五年影响因子：37.4 ）

ISSN： 1061-4036

年卷期： 2025 年 57 卷 6 期

页码：

收录情况： SCI

摘要： Resistance to wheat powdery mildew is commonly mediated by individual resistance proteins, most of which encode nucleotide-binding leucine-rich repeat (NLR) receptors. Here we report that the powdery mildew resistance gene MLIW170/PM26 in wild emmer and bread wheat derivatives is determined by a genetically linked atypical NLR pair TdCNL1/TdCNL5. Map-based cloning and PacBio HiFi long-read sequencing revealed that TdCNL1 encodes an atypical coiled-coil-domain-containing NLR protein (CNL) fused with a new potassium-dependent sodium-calcium exchanger integrated domain, whereas TdCNL5 encodes a canonical CNL protein. Mutagenesis and virus-induced gene silencing experiments indicated that both TdCNL1 and TdCNL5 are essential for powdery mildew resistance. Transgenic plants with TdCNL1 alone or TdCNL1/TdCNL5 together show resistance, whereas Fielder with TdCNL5 alone was susceptible. Geographically, MLIW170/PM26 occurs in a few Southern populations of wild emmer wheat. Our study highlights an atypical NLR pair coordinately regulating powdery mildew resistance and provides a diversified resistance gene resource for wheat improvement.

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