The Heterologous Expression of the Iris lactea var. chinensis Type 2 Metallothionein IlMT2b Gene Enhances Copper Tolerance in Arabidopsis thaliana

文献类型： 外文期刊

第一作者： Gu, Chun-Sun

作者： Gu, Chun-Sun;Huang, Su-Zhen;Lu, Xiao-Qing;Gu, Chun-Sun;Huang, Su-Zhen;Lu, Xiao-Qing;Liu, Liang-Qin;Zhu, Xu-Dong;Deng, Yan-Ming

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关键词： Iris. lactea var. chinensis;Metallothionein;Cu stress;Arabidopsis thaliana

期刊名称：BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY （ 影响因子：2.151； 五年影响因子：2.129 ）

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收录情况： SCI

摘要： Iris lactea var. chinensis (I. lactea var. chinensis) is a widely adapted perennial species with a high level of copper tolerance. To evaluate the role of metallothioneins (MTs) in copper tolerance in I. lactea var. chinensis, a full-length cDNA homologue of MT2, designated IlMT2b (GenBank accession No. AB907788), was cloned using the RACE-PCR method. The expression level of IlMT2b in the leaves and roots of I. lactea var. chinensis was induced in response to copper (Cu) treatment. Ectopic expression of IlMT2b in Arabidopsis thaliana increased the Cu concentration and reduced H2O2 production in the transgenic plants. After treatment with 50 and 100 mu M Cu, the root length of two transgenic seedlings was respectively about 1.5- and 3-fold longer than that of the wild-type. Together, these results suggested that IlMT2b may represent a useful target gene for the phytoremediation of Cu-polluted soil.

分类号： R99

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