Overexpression of Iris. lactea var. chinensis metallothionein IIMT2a enhances cadmium tolerance in Arabidopsis thaliana

文献类型： 外文期刊

第一作者： Gu, Chun-Sun

作者： Gu, Chun-Sun;Zhao, Yan-Hai;Huang, Su-Zhen;Gu, Chun-Sun;Zhao, Yan-Hai;Huang, Su-Zhen;Liu, Liang-qin;Zhu, Xu-dong;Deng, Yan-ming

作者机构：

关键词： Arabidopsis;Metallothionein;Iris. lactea var. chinensis;Cadmium stress;Reactive oxygen species

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.291； 五年影响因子：6.393 ）

ISSN： 0147-6513

年卷期： 2014 年 105 卷

页码：

收录情况： SCI

摘要： Metallothioneins (MTs) are cysteine-rich, low molecular weight, heavy metal-binding protein molecules. Here, a full-length cDNA homologue of MT2a (type 2 metallothionein) was isolated from the cadmiumtolerant species Iris. lactea var. chinensis (I. lactea var. chinensis). Expression of IIMT2a in I. lactea var. chinensis roots and leaves was up-regulated in response to cadmium stress. When the gene was constitutively expressed in Arabidopsis thaliana (A. thaliana), root length of transgenic lines was longer than that of wild-type under 50 mu M or 100 mu M cadmium stress. However, there was no difference of cadmium absorption between wild-type and trangenic lines. Histochemical staining by 3,3-diamino-benzidine (DAB) and nitroblue tetrazoliu (NBT) clearly demonstrated that transgenic lines accumulated remarkably less H2O2 and O-2(center dot-) than wild-type. Together, IIMT2a may be a promising gene for the cadmium tolerance improvement. (C) 2014 Elsevier Inc. All rights reserved.

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