Isolation and characterization of twenty polymorphic microsatellites for Hemibarbus labeo (Cyprinidae)

文献类型： 外文期刊

第一作者： Lu, Cuiyun

作者： Lu, Cuiyun;Gu, Ying;Geng, Longwu;Cheng, Lei;Li, Chao;Tong, Guangxiang;Jiang, Haifeng;Xu, Wei;Sun, Xiaowen;Mahboob, Shahid

作者机构：

关键词： Hemibarbus labeo;Microsatellite;Genetic conservation

期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Hemibarbus labeo is a valuable freshwater fish species distributed in the Heilongjiang River basin in China. Wild resources have declined sharply in recent years because of overfishing and pollution. Herein, twenty polymorphic microsatellites were isolated from an enriched genomic library in H. labeo for genetic conservation. These markers were characterized in 27 wild and 48 cultured individuals. The number of alleles per locus ranged from two to ten, with an average of 5.200. The observed heterozygosity and the expected heterozygosity varied from 0.086 to 0.870 (mean = 0.538) and from 0.116 to 0.861 (mean = 0.5441), respectively. Three and two loci significantly deviated from Hard-Weinberg equilibrium in wild and cultured individuals, respectively. Three pairwise comparisons of six loci showed significant genotypic linkage disequilibrium in cultured individuals. These polymorphic markers represent a powerful tool to study population genetic diversity, genetic conservation and breeding systems in H. labeo.

分类号： Q1

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