Development and efficacy of a novel live-attenuated QX-like nephropathogenic infectious bronchitis virus vaccine in China

文献类型: 外文期刊

第一作者: Feng, Keyu

作者: Feng, Keyu;Xue, Yu;Chen, Weiguo;Chen, Feng;Bi, Yingzuo;Xie, Qingmei;Feng, Keyu;Bi, Yingzuo;Xie, Qingmei;Chen, Weiguo;Chen, Feng;Bi, Yingzuo;Xie, Qingmei;Xue, Yu;Xie, Qingmei;Wang, Jinglan

作者机构:

关键词: Live-attenuated vaccine strain;QX-like;Nephropathogenic;Infectious bronchitis virus

期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: In this study, we attenuated a Chinese QX-like nephropathogenic infectious bronchitis virus (IBV) strain, YX10, by passaging through fertilized chicken eggs. The 90th passage strain (YX10p90) was selected as the live-attenuated vaccine candidate strain. YX10p90 was found to be safe in 7-day-old specific pathogen free chickens without induction of morbidity or mortality. YX10p90 provided nearly complete protection against QX-like (CH I genotype) strains and partial protection against other two major Chinese genotype strains. YX10p90 also showed no reversion to virulence after five back passages in chickens. An IBV polyvalent vaccine containing YX10p90 was developed and showed that it could provide better protection against major Chinese IBV virulent strains than commercial polyvalent vaccines. In addition, the complete genome sequence of YX10p90 was sequenced. Multiple-sequence alignments identified 38 nucleotide substitutions in the whole genome which resulted in 26 amino acid substitutions and a 110-bp deletion in the 3' untranslated region. In conclusion, the attenuated YX10p90 strain exhibited a fine balance between attenuation and immunogenicity, and should be considered as a candidate vaccine to prevent infection of Chinese QX-like nephropathogenic IBV. (C) 2015 Elsevier Ltd. All rights reserved.

分类号: R392

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